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Product Info Summary
| SKU: | A03496-1 |
|---|---|
| Size: | 50µg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-αB-Crystallin Antibody
View all AlphaB Crystallin/CRYAB Antibodies
SKU/Catalog Number
A03496-1
Size
50µg
Form
Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Description
Rabbit Polyclonal antibody for CRYAB detection. Tested positive for IF, IHC, WB in Human, Mouse, Rat
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-αB-Crystallin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03496-1)
Host
Rabbit
Contents
Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide corresponding to the sequence near the C-terminus of human αB-Crystallin. The sequence is completely conserved in rat, rabbit, and bovine.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03496-1 is reactive to CRYAB in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
83786 MW
Background of AlphaB Crystallin/CRYAB
Anti-Glycogen Synthase 1 pS641 antibody is validated by IHC, Western Blot and ELISA. Human muscle glycogen synthase (GS) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GS is inactivated by the serine/threonine kinase called glycogen synthase kinase-3 that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GS at S641. Phosphorylation of GS at S641 has been associated with Antiphospholipid Antibody Syndrome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03496-1 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Validation Images & Assay Conditions
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Figure 1. IHC analysis of CRYAB using anti-CRYAB antibody (A03496-1).
CRYAB was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CRYAB Antibody (A03496-1) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 2. Western blot analysis of CRYAB using anti-CRYAB antibody (A03496-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRYAB antigen affinity purified polyclonal antibody (Catalog # A03496-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for CRYAB.
Protein Target Info & Infographic
Gene/Protein Information For CRYAB (Source: Uniprot.org, NCBI)
Gene Name
CRYAB
Full Name
Alpha-crystallin B chain
Weight
83786 MW
Superfamily
small heat shock protein (HSP20) family
Alternative Names
muscle glycogen synthase pS641, glycogen synthase pS641, Glycogen, Glycogen synthase 1 (muscle), Glycogen synthase 1, Glycogen synthase1, GYS 1, GYS-1, GYS1, Starch synthase muscle CRYAB CMD1II, CRYA2, CTPP2, CTRCT16, HEL-S-101, HSPB5, MFM2 crystallin alpha B alpha-crystallin B chain|epididymis secretory protein Li 101|heat shock protein beta-5|heat-shock 20 kD like-protein|renal carcinoma NY-REN-27|rosenthal fiber component
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CRYAB, check out the CRYAB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CRYAB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-αB-Crystallin Antibody (A03496-1)
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