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Product Info Summary
| SKU: | M00720 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Alpha-1-Antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker) Monoclonal Antibody
View all Serpin A1/alpha 1-Antitrypsin Antibodies
SKU/Catalog Number
M00720
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Alpha-1-Antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker) Monoclonal Antibody (Catalog # M00720). Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Alpha-1-Antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00720)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: AAT/1379
Isotype
IgG1, kappa
Immunogen
Recombinant human Alpha-1-Antitrypsin (AAT) protein fragment
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M00720 is reactive to SERPINA1 in Human
Reconstitution
Reconstitute with distilled water.
Calculated molecular weight
46737 MW
Background of Serpin A1/alpha 1-Antitrypsin
The immunohistochemical staining of AAT is useful in identification of benign and malignant hepatic tumors and yolk sac carcinomas. Positive staining for AAT is also used in detection of benign and malignant lesions of histiocytic nature. This antibody is may also useful tool in the screening of patients with cryptogenic cirrhosis or other forms of liver disease with fibrosis of uncertain origin.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00720 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-Alpha-1-Antitrypsin Mouse Monoclonal Antibody (AAT/1379).
Protein Target Info & Infographic
Gene/Protein Information For SERPINA1 (Source: Uniprot.org, NCBI)
Gene Name
SERPINA1
Full Name
Alpha-1-antitrypsin
Weight
46737 MW
Superfamily
serpin family
Alternative Names
Alpha-1-antitrypsin;Alpha-1 protease inhibitor;Alpha-1-antiproteinase;Serpin A1;Short peptide from AAT;SPAAT;SERPINA1;AAT, PI;PRO0684, PRO2209; SERPINA1 A1A, A1AT, AAT, PI, PI1, PRO2275, alpha1AT, nNIF serpin family A member 1 alpha-1-antitrypsin|alpha-1 antitrypsin|alpha-1 protease inhibitor|alpha-1-antiproteinase|alpha-1-antitrypsin short transcript variant 1C4|alpha-1-antitrypsin short transcript variant 1C5|epididymis secretory sperm binding protein|protease inhibitor 1 (anti-elastase), alpha-1-antitrypsin|serine (or cysteine) proteinase inhibitor, clade A, member 1|serpin A1|serpin peptidase inhibitor clade A (alpha-1antiproteinase, antitrypsin) member 1|serpin peptidase inhibitor clade A member 1|serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SERPINA1, check out the SERPINA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SERPINA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Alpha-1-Antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker) Monoclonal Antibody (M00720)
Hello CJ!
M00720 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Clinical and pathological characteristics of IgG4%u2011related interstitial lung disease
Lv X, Gao F, Liu Q, Zhang S, Huang Z, Zhu Y, Zong H, Li Q, Li S. Exp Ther Med. 2018 Feb;15(2):1465-1473. doi: 10.3892/etm.2017.5554. Epub 2017 Nov 23. Clinical and pathological characteristics of IgG4-related interstitial lung disease
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Alpha-1-Antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker) Monoclonal Antibody
Question
We are interested in to test anti-Alpha-1-antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker) Monoclonal antibody M00720 on human plasma for research purposes, then I may be interested in using anti-Alpha-1-antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker) Monoclonal antibody M00720 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
The products we sell, including anti-Alpha-1-antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker) Monoclonal antibody M00720, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-16
Question
I was wanting to use your anti-Alpha-1-antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker) Monoclonal antibody for IHC for human plasma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human plasma identification?
Verified Customer
Verified customer
Asked: 2019-07-30
Answer
As indicated on the product datasheet, M00720 anti-Alpha-1-antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker) Monoclonal antibody has been tested for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human plasma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-30
Question
Does anti-Alpha-1-antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker) Monoclonal antibody M00720 work on monkey IHC with brain cajal-retzius cell?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
Our lab technicians have not validated anti-Alpha-1-antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker) Monoclonal antibody M00720 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Alpha-1-antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker) Monoclonal antibody M00720 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey brain cajal-retzius cell in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-11
Question
Would anti-Alpha-1-antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker) Monoclonal antibody M00720 work for IHC with plasma?
N. Wu
Verified customer
Asked: 2018-07-02
Answer
According to the expression profile of plasma, SERPINA1 is highly expressed in plasma. So, it is likely that anti-Alpha-1-antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker) Monoclonal antibody M00720 will work for IHC with plasma.
Boster Scientific Support
Answered: 2018-07-02
Question
I see that the anti-Alpha-1-antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker) Monoclonal antibody M00720 works with IHC, what is the protocol used to produce the result images on the product page?
H. Miller
Verified customer
Asked: 2017-02-13
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-02-13
Question
Do you have a BSA free version of anti-Alpha-1-antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker) Monoclonal antibody M00720 available?
T. Li
Verified customer
Asked: 2015-06-23
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Alpha-1-antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker) Monoclonal antibody M00720 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-06-23
Question
Here is the WB image, lot number and protocol we used for plasma using anti-Alpha-1-antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker) Monoclonal antibody M00720. Please let me know if you require anything else.
J. Singh
Verified customer
Asked: 2015-02-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-02-23
