Anti-Alpha-1-Trypsin SERPINA1 Antibody Peroxidase Conjugated

Product Name

Anti-Alpha-1-Trypsin SERPINA1 Antibody Peroxidase Conjugated

View all Serpin A1/alpha 1-Antitrypsin Antibodies

SKU/Catalog Number

H00720

Size

100ug

Form

Lyophilized

Description

Boster Bio Anti-Alpha-1-Trypsin SERPINA1 Antibody Peroxidase Conjugated (Catalog # H00720). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Alpha-1-Trypsin SERPINA1 Antibody Peroxidase Conjugated (Boster Biological Technology, Pleasanton CA, USA, Catalog # H00720)

Host

Goat

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Clonality

Polyclonal

Isotype

IgG

Immunogen

Anti-alpha 1 Anti-Trypsin Antibody was produced by repeated immunizations with native α1-Anti-Trypsin protein from human plasma.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

H00720 is reactive to SERPINA1 in Human

Applications

H00720 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

46737 MW

Background of Serpin A1/alpha 1-Antitrypsin

Anti-Alpha 1-Anti-Trypsin Antibody detects a1-Anti-Trypsin protein. a1-Anti-Trypsin is an inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Anti-Trypsin irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form of Antitrypsin inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Defects in SERPINA1 are the cause emphysema, liver cirrhosis and liver failure. Environmental factors, particularly cigarette smoking, greatly increase the risk of emphysema at an earlier age. Anti-Alpha-1-Anti-Trypsin Antibody is ideal for investigators involved in Cell Signaling, Signal Transduction and Cancer research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:1,000 - 1:5,000
IHC: 1:200 - 1:1,000
WB: 1:500 - 1:2,500

Validation Images & Assay Conditions

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Western blot analysis of Alpha-1-Anti-Trypsin expression in reduced Alpha-1-Anti-Trypsin using peroxidase conjugated goat anti-Alpha-1-Anti-Trypsin polyclonal antibody (Catalog # H00720) at 1:3000. Load: 1

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Western Blot analysis of peroxidase conjugated goat anti-Alpha-1-Anti-Trypsin polyclonal antibody (Catalog # H00720) at 1:400. Lane 1: molecular weight. Lane 2: Goat-anti-Alpha-1-Anti-Trypsin (red), Rabbit anti-Transferrin, and Mouse-a-GST. Load: 35

Gene/Protein Information For SERPINA1 (Source: Uniprot.org, NCBI)

Alternative Names

A1A; A1AT; AATMGC23330; alpha 1-Antitrypsin; alpha 1-Proteinase Inhibitor; Alpha-1 protease inhibitor; Alpha-1-antiproteinase; alpha-1-antitrypsin; antitrypsin), member 1; member 1; PIMGC9222; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase; serine (or cysteine) proteinase inhibitor, clade A, member 1; Serpin A1; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin) SERPINA1 A1A, A1AT, AAT, PI, PI1, PRO2275, alpha1AT, nNIF serpin family A member 1 alpha-1-antitrypsin|alpha-1 antitrypsin|alpha-1 protease inhibitor|alpha-1-antiproteinase|alpha-1-antitrypsin short transcript variant 1C4|alpha-1-antitrypsin short transcript variant 1C5|epididymis secretory sperm binding protein|protease inhibitor 1 (anti-elastase), alpha-1-antitrypsin|serine (or cysteine) proteinase inhibitor, clade A, member 1|serpin A1|serpin peptidase inhibitor clade A (alpha-1antiproteinase, antitrypsin) member 1|serpin peptidase inhibitor clade A member 1|serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1

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In this infographic, you will see the following information for SERPINA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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