Product Info Summary
SKU: | P06115 |
---|---|
Size: | 100ug |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Alkaline Phosphatase ALPI Antibody
View all Alkaline Phosphatase, Intestinal Antibodies
SKU/Catalog Number
P06115
Size
100ug
Form
Lyophilized
Description
Boster Bio Anti-Alkaline Phosphatase ALPI Antibody (Catalog # P06115). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.?Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Alkaline Phosphatase ALPI Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P06115)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
Alkaline Phosphatase [Human Intestine]
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P06115 is reactive to ALP1 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Observed Molecular Weight
52 kDa
Calculated molecular weight
56812 MW
Background of Alkaline Phosphatase, Intestinal
In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney). Alkaline Phosphatase catalytic activity is phosphate monoester + H2O = an alcohol + phosphate.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P06115 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:4,000 - 1:20,000
WB: 1:500 - 1:2,000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Alkaline Phosphatase expression in cellular extracts. Alkaline Phosphatase at 60KD was detected using rabbit anti-Alkaline Phosphatase Antigen Affinity purified polyclonal antibody (Catalog # P06115) at 1:5000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Click image to see more details
Western blot analysis of Alkaline Phosphatase expression in Alkaline Phosphatase recombinant protein. Alkaline Phosphatase at 58KD was detected using rabbit anti-Alkaline Phosphatase Antigen Affinity purified polyclonal antibody (Catalog # P06115) at 1:1000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Protein Target Info & Infographic
Gene/Protein Information For ALP1 (Source: Uniprot.org, NCBI)
Gene Name
ALP1
Full Name
Intestinal-type alkaline phosphatase
Weight
56812 MW
Superfamily
alkaline phosphatase family
Alternative Names
Alkaline Phosphatase, Alkaline phosphatase intestinal, Alkaline phosphomonoesterase, ALPI, Glycerophosphatase, IAP, Intestinal alkaline phosphatase, Kasahara isozyme ALPI IAP alkaline phosphatase, intestinal intestinal-type alkaline phosphatase|Kasahara isozyme|alkaline phosphomonoesterase|glycerophosphatase|intestinal alkaline phosphatase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ALP1, check out the ALP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ALP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Alkaline Phosphatase ALPI Antibody (P06115)
Hello CJ!
P06115 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Synergistic effects of fibroblast growth factor-2 and bone morphogenetic protein-2 on bone induction
Synergistic effects of fibroblast growth factor-2 and bone morphogenetic protein-2 on bone induction
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Customer Q&As
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3 Customer Q&As for Anti-Alkaline Phosphatase ALPI Antibody
Question
We are currently using anti-Alkaline Phosphatase antibody P06115 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2017-11-27
Answer
The anti-Alkaline Phosphatase antibody (P06115) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-27
Question
Will anti-Alkaline Phosphatase antibody P06115 work on canine WB with cervix carcinoma?
S. Wu
Verified customer
Asked: 2016-10-05
Answer
Our lab technicians have not validated anti-Alkaline Phosphatase antibody P06115 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Alkaline Phosphatase antibody P06115 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-10-05
Question
Will P06115 anti-Alkaline Phosphatase antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
P. Wu
Verified customer
Asked: 2015-03-16
Answer
You can see on the product datasheet, P06115 anti-Alkaline Phosphatase antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-03-16