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Product Info Summary
| SKU: | M00301-1 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-ALK Rabbit Monoclonal Antibody, Clone#RM361
SKU/Catalog Number
M00301-1
Size
100uL
Form
Liquid
Description
Boster Bio Anti-ALK Rabbit Monoclonal Antibody, Clone#RM361 (Catalog # M00301-1). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ALK Rabbit Monoclonal Antibody, Clone#RM361 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00301-1)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM361
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the C-terminus of human ALK (Anaplastic lymphoma kinase)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts human total ALK protein including ALK fusion proteins
Reactive Species
M00301-1 is reactive to ALK in Human
Reconstitution
Calculated molecular weight
176442 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00301-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:500-1:1000 dilution
WB: 1:1000-1:2000 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of NSCLC cell line H2228 (Cell Lysate) expressing EML4-ALK variant 3, using anti-ALK rabbit monoclonal antibody (Clone RM361) at a 1:2000 dilution.
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Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded NCI-H2228 cells (expressing EML4-ALK variant 3) and Daudi cells (ALK negative), stained with anti-ALK rabbit monoclonal antibody clone RM361.
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Figure 3. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human brain tissue section using anti-ALK rabbit monoclonal antibody (Clone RM361) at a 1:1000 dilution.
Protein Target Info & Infographic
Gene/Protein Information For ALK (Source: Uniprot.org, NCBI)
Gene Name
ALK
Full Name
ALK tyrosine kinase receptor
Weight
176442 MW
Superfamily
protein kinase superfamily
Alternative Names
ALK tyrosine kinase receptor;2.7.10.1;Anaplastic lymphoma kinase;CD246;ALK; ALK CD246, NBLST3 ALK receptor tyrosine kinase ALK tyrosine kinase receptor|CD246 |anaplastic lymphoma receptor tyrosine kinase|mutant anaplastic lymphoma kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ALK, check out the ALK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ALK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ALK Rabbit Monoclonal Antibody, Clone#RM361 (M00301-1)
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-ALK Rabbit Monoclonal Antibody, Clone#RM361
Question
Can you help my question with product M00301-1, anti-ALK Rabbit Monoclonal antibody, Clone#RM361. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00301-1 anti-ALK Rabbit Monoclonal antibody, Clone#RM361, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-14
Question
My boss were happy with the WB result of your anti-ALK Rabbit Monoclonal antibody, Clone
R. Li
Verified customer
Asked: 2020-04-02
Answer
According to literature, brain does express ALK. Generally ALK expresses in cell membrane. Regarding which tissues have ALK expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15815621
Boster Scientific Support
Answered: 2020-04-02
Question
I see that the anti-ALK Rabbit Monoclonal antibody, Clone#RM361 M00301-1 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-23
Question
We are currently using anti-ALK Rabbit Monoclonal antibody, Clone#RM361 M00301-1 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
The anti-ALK Rabbit Monoclonal antibody, Clone#RM361 (M00301-1) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-20
Question
I was wanting to use your anti-ALK Rabbit Monoclonal antibody, Clone#RM361 for IHC for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
It shows on the product datasheet, M00301-1 anti-ALK Rabbit Monoclonal antibody, Clone#RM361 has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-31
Question
We bought anti-ALK Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
I have checked the website and datasheets of our anti-ALK Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2019-07-26
Question
Will anti-ALK Rabbit Monoclonal antibody, Clone#RM361 M00301-1 work for IHC with brain?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
According to the expression profile of brain, ALK is highly expressed in brain. So, it is likely that anti-ALK Rabbit Monoclonal antibody, Clone#RM361 M00301-1 will work for IHC with brain.
Boster Scientific Support
Answered: 2019-06-27
Question
Is a blocking peptide available for product anti-ALK Rabbit Monoclonal antibody, Clone#RM361 (M00301-1)?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
We do provide the blocking peptide for product anti-ALK Rabbit Monoclonal antibody, Clone#RM361 (M00301-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-21
Question
Is this M00301-1 anti-ALK Rabbit Monoclonal antibody, Clone#RM361 reactive to the isotypes of ALK?
N. Anderson
Verified customer
Asked: 2019-04-12
Answer
The immunogen of M00301-1 anti-ALK Rabbit Monoclonal antibody, Clone#RM361 is A peptide corresponding to the C-terminus of human ALK (Anaplastic lymphoma kinase). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-04-12
Question
Do you have a BSA free version of anti-ALK Rabbit Monoclonal antibody, Clone#RM361 M00301-1 available?
Verified Customer
Verified customer
Asked: 2019-03-21
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ALK Rabbit Monoclonal antibody, Clone#RM361 M00301-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-03-21
Question
My lab would like to test anti-ALK Rabbit Monoclonal antibody, Clone#RM361 M00301-1 on human brain for research purposes, then I may be interested in using anti-ALK Rabbit Monoclonal antibody, Clone#RM361 M00301-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-12-21
Answer
The products we sell, including anti-ALK Rabbit Monoclonal antibody, Clone#RM361 M00301-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-12-21
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-ALK Rabbit Monoclonal antibody, Clone#RM361 M00301-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-06-20
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-20
Question
See attached the WB image, lot number and protocol we used for brain using anti-ALK Rabbit Monoclonal antibody, Clone#RM361 M00301-1. Please let me know if you require anything else.
K. Rodriguez
Verified customer
Asked: 2017-03-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-03-07
Question
Would M00301-1 anti-ALK Rabbit Monoclonal antibody, Clone#RM361 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
H. Baker
Verified customer
Asked: 2014-11-10
Answer
It shows on the product datasheet, M00301-1 anti-ALK Rabbit Monoclonal antibody, Clone#RM361 as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-11-10
Question
We have observed staining in human sperm. Are there any suggestions? Is anti-ALK Rabbit Monoclonal antibody, Clone#RM361 supposed to stain sperm positively?
J. Jackson
Verified customer
Asked: 2013-12-26
Answer
From what I have seen in literature sperm does express ALK. From what I have seen in Uniprot.org, ALK is expressed in sperm, brain, among other tissues. Regarding which tissues have ALK expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15815621
Boster Scientific Support
Answered: 2013-12-26
Question
My question regards using your anti-ALK Rabbit Monoclonal antibody, Clone#RM361 for phosphorylation studies. Has this antibody been tested with western blotting on daudi cells? We would like to see some validation images before ordering.
T. Parker
Verified customer
Asked: 2013-01-14
Answer
We appreciate your inquiry. This M00301-1 anti-ALK Rabbit Monoclonal antibody, Clone#RM361 is validated on daudi cells, human brain tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-01-14
