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Product Info Summary
| SKU: | A07963 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, ICC |
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Product info
Product Name
Anti-A-kinase anchor protein 11 AKAP11 Antibody
SKU/Catalog Number
A07963
Size
100ul
Form
Liquid
Description
Boster Bio Anti-A-kinase anchor protein 11 AKAP11 Antibody (Catalog# A07963). Tested in IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Rat, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-A-kinase anchor protein 11 AKAP11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07963)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human AKAP11. Immunogen sequence location: 1761-1810
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07963 is reactive to AKAP11 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
210512 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07963 is guaranteed for ELISA, IF, IHC-P, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:10000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunofluorescence analysis of HepG2 cells, using AKAP11 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using AKAP11 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For AKAP11 (Source: Uniprot.org, NCBI)
Gene Name
AKAP11
Full Name
A-kinase anchor protein 11
Weight
210512 MW
Superfamily
AKAP110 family
Alternative Names
AKAP11;AKAP220;KIAA0629;A-kinase anchor protein 11;AKAP-11;A-kinase anchor protein 220 kDa;AKAP 220;hAKAP220;Protein kinase A-anchoring protein 11;PRKA11 AKAP11 AKAP-11, AKAP220, PPP1R44, PRKA11 A-kinase anchoring protein 11 A-kinase anchor protein 11|A kinase (PRKA) anchor protein 11|A-kinase anchor protein 220 kDa|A-kinase anchoring protein, 220kDa|a kinase anchor protein 220 kDa|protein kinase A anchoring protein 11|protein phosphatase 1, regulatory subunit 44
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AKAP11, check out the AKAP11 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AKAP11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-A-kinase anchor protein 11 AKAP11 Antibody (A07963)
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Customer Q&As
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6 Customer Q&As for Anti-A-kinase anchor protein 11 AKAP11 Antibody
Question
We need to test anti-AKAP11 antibody A07963 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-AKAP11 antibody A07963 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
The products we sell, including anti-AKAP11 antibody A07963, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-22
Question
Would anti-AKAP11 antibody A07963 work for IF with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
According to the expression profile of cervix carcinoma erythroleukemia, AKAP11 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-AKAP11 antibody A07963 will work for IF with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2019-09-16
Question
Does A07963 anti-AKAP11 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Anderson
Verified customer
Asked: 2018-11-14
Answer
As indicated on the product datasheet, A07963 anti-AKAP11 antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-11-14
Question
We are currently using anti-AKAP11 antibody A07963 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-31
Answer
The anti-AKAP11 antibody (A07963) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-31
Question
I see that the anti-AKAP11 antibody A07963 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-08-15
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-08-15
Question
Is this A07963 anti-AKAP11 antibody reactive to the isotypes of AKAP11?
Verified Customer
Verified customer
Asked: 2018-01-29
Answer
The immunogen of A07963 anti-AKAP11 antibody is A peptide derived from human AKAP11. Immunogen sequence location: 1761-1810. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-01-29
