Anti-Aryl hydrocarbon Receptor/AHR Antibody Picoband®

AHR antibody

Boster Bio Anti-Aryl hydrocarbon Receptor/AHR Antibody Picoband® catalog # PB9419. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 1 publication(s).

Product Info Summary

SKU: PB9419
Size: 100 μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Aryl hydrocarbon Receptor/AHR Antibody Picoband®

View all AHR Antibodies

SKU/Catalog Number

PB9419

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Aryl hydrocarbon Receptor/AHR Antibody Picoband® catalog # PB9419. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Aryl hydrocarbon Receptor/AHR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9419)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of mouse AHR, different from the related human sequence by five amino acids, and from the related rat sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9419 is reactive to Ahr in Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

96 kDa

Calculated molecular weight

95017 MW

Background of AHR

AHR (aryl hydrocarbon receptor), also called bHLHe76, is a member of the family of basic helix-loop-helix transcription factors. AhR is a cytosolic transcription factor that is normally inactive, bound to several co-chaperones. The AHR gene is mapped on 7p21.1. Estrogenic actions of AHR agonists were detected in wildtype ovariectomized mouse uteri, but were absent in Ahr -/- or Er-alpha -/- ovariectomized mice. Complex assembly and ubiquitin ligase activity of CUL4B (AHR) in vitro and in vivo are dependent on the AHR ligand. In the CUL4B (AHR) complex, ligand-activated AHR acts as a substrate-specific adaptor component that targets sex steroid receptors for degradation. Cd4-positive cells from mice lacking Ahr developed Th17 responses but failed to produce Il22 and did not show enhanced Th17 development. Activation of Ahr during induction of EAE accelerated disease onset and increased pathology in wildtype mice, but not in Ahr -/- mice. The TDO-AHR pathway is active in human brain tumors and is associated with malignant progression and poor survival. Ahr activity within ROR-gamma-t-positive ILC could be induced by dietary ligands such as those contained in vegetables of the family Brassicaceae.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PB9419 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Rat

Positive Control

WB: Rat Brain Tissue, Mouse Liver Tissue

Validation Images & Assay Conditions

Gene/Protein Information For Ahr (Source: Uniprot.org, NCBI)

Gene Name

Ahr

Full Name

Aryl hydrocarbon receptor

Weight

95017 MW

Alternative Names

Aryl hydrocarbon receptor;Ah receptor;AhR;Ahr; AHR RP85, bHLHe76 aryl hydrocarbon receptor aryl hydrocarbon receptor|AH-receptor|ah receptor|aromatic hydrocarbon receptor|class E basic helix-loop-helix protein 76

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Ahr, check out the Ahr Infographic

Ahr infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Ahr: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9419 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Yang X,Liu H,Ye T,Duan C,Lv P,Wu X,Liu J,Jiang K,Lu H,Yang H,Xia D,Peng E,Chen Z,Tang K,Ye Z. AhR activation attenuates calcium oxalate nephrocalcinosis by diminishing M1 macrophage polarization and promoting M2 macrophage polarization. Theranostics.2020
Species: Mouse
PB9419 usage in article: APP:WB, SAMPLE:BMDMS, DILUTION:1:200

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4 Customer Q&As for Anti-Aryl hydrocarbon Receptor/AHR Antibody Picoband®

Question

We are currently using anti-Aryl hydrocarbon Receptor/AHR antibody PB9419 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-23

Answer

The anti-Aryl hydrocarbon Receptor/AHR antibody (PB9419) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-23

Question

We have seen staining in mouse liver. Any tips? Is anti-Aryl hydrocarbon Receptor/AHR antibody supposed to stain liver positively?

Verified Customer

Verified customer

Asked: 2020-01-13

Answer

According to literature liver does express AHR. According to Uniprot.org, AHR is expressed in placenta, liver, among other tissues. Regarding which tissues have AHR expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 8393992
Placenta, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-01-13

Question

Our team were content with the WB result of your anti-Aryl hydrocarbon Receptor/AHR antibody. However we have seen positive staining in placenta cytoplasm. using this antibody. Is that expected? Could you tell me where is AHR supposed to be expressed?

H. Baker

Verified customer

Asked: 2019-01-30

Answer

According to literature, placenta does express AHR. Generally AHR expresses in cytoplasm. Regarding which tissues have AHR expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 8393992
Placenta, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-01-30

Question

My lab would like using your anti-Aryl hydrocarbon Receptor/AHR antibody for circumferential growth involved in left ventricle morphogenesis studies. Has this antibody been tested with western blotting on rat brain tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2017-05-19

Answer

Thanks for your inquiry. This PB9419 anti-Aryl hydrocarbon Receptor/AHR antibody is tested on rat brain tissue, tissue lysate, mouse liver tissue. It is guaranteed to work for WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-05-19

Order DetailsPrice
PB9419

100μg

$370
PB9419-10ug

10μg sample (liquid)

$99
PB9419-Biotin

100 μg Biotin conjugated

$570
PB9419-Cy3

100 μg Cy3 conjugated

$570
PB9419-Dylight488

100 μg Dylight488 conjugated

$570
PB9419-Dylight550

100 μg Dylight550 conjugated

$570
PB9419-Dylight594

100 μg Dylight594 conjugated

$570
PB9419-FITC

100 μg FITC conjugated

$570
PB9419-HRP

100 μg HRP conjugated

$570
PB9419-APC

100 μg APC conjugated

$670
PB9419-PE

100 μg PE conjugated

$670
PB9419-iFluor647

100 μg iFluor647 conjugated

$670
PB9419-carrier-free

Carrier Free

$370

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PB9419
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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