Product Info Summary
SKU: | A02590-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-AF4 AFF1 Antibody
SKU/Catalog Number
A02590-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-AF4 AFF1 Antibody (Catalog# A02590-1). Tested in IHC-P, IF (paraffin), WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AF4 AFF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02590-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Standard Protein
A peptide derived from human AF4. Immunogen sequence location: 1-50
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A02590-1 is reactive to AFF1 in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
72 kDa
Calculated molecular weight
131422 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02590-1 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500-2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:20000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using AF4 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For AFF1 (Source: Uniprot.org, NCBI)
Gene Name
AFF1
Full Name
AF4/FMR2 family member 1
Weight
131422 MW
Superfamily
AF4 family
Alternative Names
AF-4; AF4/FMR2 family member 1; AF4/FMR2 family, member 1; AF4myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 2; ALL1-fused gene from chromosome 4 protein; FEL; MLLT2MGC134969; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 2; PBM1; pre-B-cell monocytic leukemia partner 1; Protein AF-4; Protein FEL; Proto-oncogene AF4 AFF1 AF4, MLLT2, PBM1 AF4/FMR2 family member 1 AF4/FMR2 family member 1|ALL1-fused gene from chromosome 4 protein|myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 2|pre-B-cell monocytic leukemia partner 1|proto-oncogene AF4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AFF1, check out the AFF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AFF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AF4 AFF1 Antibody (A02590-1)
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No publications found for A02590-1
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Customer Q&As
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7 Customer Q&As for Anti-AF4 AFF1 Antibody
Question
Would A02590-1 anti-AF4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
As indicated on the product datasheet, A02590-1 anti-AF4 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-05
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-AF4 antibody A02590-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-30
Question
We are currently using anti-AF4 antibody A02590-1 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
The anti-AF4 antibody (A02590-1) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-26
Question
Will anti-AF4 antibody A02590-1 work for IHC with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
According to the expression profile of cervix carcinoma erythroleukemia, AFF1 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-AF4 antibody A02590-1 will work for IHC with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2019-09-19
Question
Is a blocking peptide available for product anti-AF4 antibody (A02590-1)?
F. Collins
Verified customer
Asked: 2017-12-12
Answer
We do provide the blocking peptide for product anti-AF4 antibody (A02590-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-12-12
Question
I was wanting to use your anti-AF4 antibody for IHC for mouse cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse cervix carcinoma erythroleukemia identification?
R. Taylor
Verified customer
Asked: 2017-01-05
Answer
As indicated on the product datasheet, A02590-1 anti-AF4 antibody has been tested for IHC on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-01-05
Question
Here is the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-AF4 antibody A02590-1. Please let me know if you require anything else.
G. Wu
Verified customer
Asked: 2015-11-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-11-24