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Product Info Summary
| SKU: | PB9001 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Adiponectin/ADIPOQ Antibody Picoband®
View all Adiponectin/Acrp30 Antibodies
SKU/Catalog Number
PB9001
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Adiponectin/ADIPOQ Antibody Picoband® catalog # PB9001. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Adiponectin/ADIPOQ Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9001)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human adiponectin recombinant protein (Position: E19-N244). Human adiponectin shares 85% amino acid (aa) sequence identity with mouse adiponectin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9001 is reactive to ADIPOQ in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
30 kDa
Calculated molecular weight
26414 MW
Background of Adiponectin/Acrp30
ADIPOQ (Adipocyte-, C1q-, and Collagen Domain-Containing),also known as APM1, ADPN or ACDC, is a protein which in humans is encoded by the ADIPOQ gene. Using FISH, Das et al. (2001) mapped the mouse Acrp30 gene to chromosome 16 in a region showing homology of synteny with human 3q27. By RNase protection and Western blot analysis, Schaffler et al. (1999) showed that APM1 is expressed by differentiated adipocytes as a 33-kD protein that is also detectable in serum. By sequence comparisons, they found links between APM1 and TNF family ligands as well as to cytokines expressed by T cells. Adiponectin is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation. Adiponectin is exclusively secreted from adipose tissue (and also from the placenta in pregnancy) into the bloodstream and is very abundant in plasma relative to many hormones.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9001 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human placenta tissue, human Hela whole cell, human Jurkat whole cell
IHC: human lung cancer tissue, human mammary cancer tissue, human placenta tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of Adiponectin using anti-Adiponectin antibody (PB9001).
Adiponectin was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Adiponectin Antibody (PB9001) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 2. IHC analysis of Adiponectin using anti-Adiponectin antibody (PB9001).
Adiponectin was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Adiponectin Antibody (PB9001) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Adiponectin using anti-Adiponectin antibody (PB9001).
Adiponectin was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Adiponectin Antibody (PB9001) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. Western blot analysis of Adiponectin using anti-Adiponectin antibody (PB9001).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Adiponectin antigen affinity purified polyclonal antibody (Catalog # PB9001) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Adiponectin at approximately 30 kDa. The expected band size for Adiponectin is at 30 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ADIPOQ (Source: Uniprot.org, NCBI)
Gene Name
ADIPOQ
Full Name
Adiponectin
Weight
26414 MW
Alternative Names
Adiponectin;30 kDa adipocyte complement-related protein;Adipocyte complement-related 30 kDa protein;ACRP30;Adipocyte, C1q and collagen domain-containing protein;Adipose most abundant gene transcript 1 protein;apM-1;Gelatin-binding protein;ADIPOQ;ACDC, ACRP30, APM1, GBP28; ADIPOQ ACDC, ACRP30, ADIPQTL1, ADPN, APM-1, APM1, GBP28 adiponectin, C1Q and collagen domain containing adiponectin|30 kDa adipocyte complement-related protein|adipocyte complement-related 30 kDa protein|adipose most abundant gene transcript 1 protein|adipose specific collagen-like factor|gelatin-binding protein 28
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ADIPOQ, check out the ADIPOQ Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ADIPOQ: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Adiponectin/ADIPOQ Antibody Picoband® (PB9001)
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Customer Q&As
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16 Customer Q&As for Anti-Adiponectin/ADIPOQ Antibody Picoband®
Question
We ordered your anti-Adiponectin/ADIPOQ antibody for IHC on adipose tissue in a previous project. I am using human, and We are going to use the antibody for WB next. you antibody examining adipose tissue as well as adipose tissue of abdominal region in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
I viewed the website and datasheets of our anti-Adiponectin/ADIPOQ antibody and it seems that PB9001 has been validated on human in both IHC and WB. Thus PB9001 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-04-03
Question
Please see the WB image, lot number and protocol we used for adipose tissue of abdominal region using anti-Adiponectin/ADIPOQ antibody PB9001. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-03
Question
I was wanting to use to test anti-Adiponectin/ADIPOQ antibody PB9001 on human adipose tissue of abdominal region for research purposes, then I may be interested in using anti-Adiponectin/ADIPOQ antibody PB9001 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
The products we sell, including anti-Adiponectin/ADIPOQ antibody PB9001, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-30
Question
Would PB9001 anti-Adiponectin/ADIPOQ antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
You can see on the product datasheet, PB9001 anti-Adiponectin/ADIPOQ antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-29
Question
My team were well pleased with the WB result of your anti-Adiponectin/ADIPOQ antibody. However we have observed positive staining in adipose tissue of abdominal region secreted. using this antibody. Is that expected? Could you tell me where is ADIPOQ supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
Based on literature, adipose tissue of abdominal region does express ADIPOQ. Generally ADIPOQ expresses in secreted. Regarding which tissues have ADIPOQ expression, here are a few articles citing expression in various tissues:
Adipose tissue, Pubmed ID: 8619847
Boster Scientific Support
Answered: 2019-07-26
Question
We are currently using anti-Adiponectin/ADIPOQ antibody PB9001 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
The anti-Adiponectin/ADIPOQ antibody (PB9001) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-04
Question
I have a question about product PB9001, anti-Adiponectin/ADIPOQ antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9001 anti-Adiponectin/ADIPOQ antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-04
Question
I am looking for using your anti-Adiponectin/ADIPOQ antibody for negative regulation of macrophage derived foam cell differentiation studies. Has this antibody been tested with western blotting on lung cancer tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
Thanks for your inquiry. This PB9001 anti-Adiponectin/ADIPOQ antibody is tested on human placenta tissue, lung cancer tissue, tissue lysate, mammary cancer tissue, hela whole cell lysate, jurkat whole cell lysate. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-07-02
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adipose tissue of abdominal region using anti-Adiponectin/ADIPOQ antibody PB9001. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-02-04
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-04
Question
Will anti-Adiponectin/ADIPOQ antibody PB9001 work for WB with adipose tissue of abdominal region?
M. Collins
Verified customer
Asked: 2018-08-28
Answer
According to the expression profile of adipose tissue of abdominal region, ADIPOQ is highly expressed in adipose tissue of abdominal region. So, it is likely that anti-Adiponectin/ADIPOQ antibody PB9001 will work for WB with adipose tissue of abdominal region.
Boster Scientific Support
Answered: 2018-08-28
Question
Is a blocking peptide available for product anti-Adiponectin/ADIPOQ antibody (PB9001)?
Verified Customer
Verified customer
Asked: 2018-07-10
Answer
We do provide the blocking peptide for product anti-Adiponectin/ADIPOQ antibody (PB9001). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-07-10
Question
I see that the anti-Adiponectin/ADIPOQ antibody PB9001 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-05-31
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-05-31
Question
We have been able to see staining in human adipose tissue of abdominal region. What should we do? Is anti-Adiponectin/ADIPOQ antibody supposed to stain adipose tissue of abdominal region positively?
G. Lewis
Verified customer
Asked: 2017-12-21
Answer
According to literature adipose tissue of abdominal region does express ADIPOQ. According to Uniprot.org, ADIPOQ is expressed in adipose tissue of abdominal region, adipose tissue, among other tissues. Regarding which tissues have ADIPOQ expression, here are a few articles citing expression in various tissues:
Adipose tissue, Pubmed ID: 8619847
Boster Scientific Support
Answered: 2017-12-21
Question
Is this PB9001 anti-Adiponectin/ADIPOQ antibody reactive to the isotypes of ADIPOQ?
Verified Customer
Verified customer
Asked: 2017-12-04
Answer
The immunogen of PB9001 anti-Adiponectin/ADIPOQ antibody is E.coli-derived human adiponectin recombinant protein (Position: E19-N244). Human adiponectin shares 85% amino acid (aa) sequence identity with mouse adiponectin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-04
Question
I was wanting to use your anti-Adiponectin/ADIPOQ antibody for WB for human adipose tissue of abdominal region on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human adipose tissue of abdominal region identification?
B. Patel
Verified customer
Asked: 2017-01-10
Answer
You can see on the product datasheet, PB9001 anti-Adiponectin/ADIPOQ antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human adipose tissue of abdominal region in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-01-10
Question
Do you have a BSA free version of anti-Adiponectin/ADIPOQ antibody PB9001 available?
R. Miller
Verified customer
Asked: 2013-10-16
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Adiponectin/ADIPOQ antibody PB9001 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-10-16
