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Product Info Summary
| SKU: | A12790 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Transcription factor IIIA GTF3A Antibody
SKU/Catalog Number
A12790
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Transcription factor IIIA GTF3A Antibody (Catalog # A12790). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Transcription factor IIIA GTF3A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12790)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from Internal of human GTF3A.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12790 is reactive to GTF3A in Human
Reconstitution
Calculated molecular weight
41515 MW
Background of GTF3A
Interacts with the internal control region (ICR) of approximately 50 bases within the 5S RNA genes, is required for correct transcription of these genes by RNA polymerase III. Also binds the transcribed 5S RNA's. May initiate transcription of the 5S ribosomal RNA gene and maintain the stability of transcription of other genes.
Arakawa H., Cytogenet. Cell Genet. 70:235-238(1995).
Dunham A., Nature 428:522-528(2004).
Drew P.D., Gene 159:215-218(1995).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12790 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from Jurkat cells, using GTF3A antibody A12790.
Protein Target Info & Infographic
Gene/Protein Information For GTF3A (Source: Uniprot.org, NCBI)
Gene Name
GTF3A
Full Name
Transcription factor IIIA
Weight
41515 MW
Alternative Names
Transcription factor IIIA;TFIIIA;GTF3A; GTF3A AP2, TFIIIA general transcription factor IIIA transcription factor IIIA
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GTF3A, check out the GTF3A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GTF3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Transcription factor IIIA GTF3A Antibody (A12790)
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No publications found for A12790
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Customer Q&As
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5 Customer Q&As for Anti-Transcription factor IIIA GTF3A Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for parotid gland using anti-GTF3A antibody A12790. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-10
Question
Would A12790 anti-GTF3A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
As indicated on the product datasheet, A12790 anti-GTF3A antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-02
Question
I was wanting to use your anti-GTF3A antibody for WB for human parotid gland on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human parotid gland identification?
D. Baker
Verified customer
Asked: 2017-11-09
Answer
It shows on the product datasheet, A12790 anti-GTF3A antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human parotid gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-11-09
Question
We are currently using anti-GTF3A antibody A12790 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2017-08-22
Answer
The anti-GTF3A antibody (A12790) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-22
Question
Does anti-GTF3A antibody A12790 work on bovine WB with brain?
P. Krishna
Verified customer
Asked: 2015-03-23
Answer
Our lab technicians have not validated anti-GTF3A antibody A12790 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-GTF3A antibody A12790 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-03-23
