Product Info Summary
SKU: | A10261 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-ACOT2 Antibody
SKU/Catalog Number
A10261
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-ACOT2 Antibody (Catalog # A10261). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ACOT2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10261)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human ACOT2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10261 is reactive to ACOT2 in Human
Reconstitution
Calculated molecular weight
53218 MW
Background of ACOT2
Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Displays high levels of activity on medium- and long chain acyl CoAs.
Sherrington R., Nature 375:754-760(1995).
Hunt M.C., FASEB J. 20:1855-1864(2006).
Ota T., Nat. Genet. 36:40-45(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10261 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from Jurkat cells and HuvEc cells, using ACOT2 antibody A10261.
Protein Target Info & Infographic
Gene/Protein Information For ACOT2 (Source: Uniprot.org, NCBI)
Gene Name
ACOT2
Full Name
Acyl-coenzyme A thioesterase 2, mitochondrial
Weight
53218 MW
Superfamily
C/M/P thioester hydrolase family
Alternative Names
Acyl-coenzyme A thioesterase 2, mitochondrial;Acyl-CoA thioesterase 2;3.1.2.2;Acyl-coenzyme A thioester hydrolase 2a;CTE-Ia;Long-chain acyl-CoA thioesterase 2;ZAP128;ACOT2;PTE2, PTE2A; ACOT2 CTE-IA, CTE1A, MTE1, PTE2, PTE2A, ZAP128 acyl-CoA thioesterase 2 acyl-coenzyme A thioesterase 2, mitochondrial|acyl-coenzyme A thioester hydrolase 2a|long-chain acyl-CoA thioesterase 2|mitochondrial acyl-CoA thioesterase 1|peroxisomal long-chain acyl-coA thioesterase 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACOT2, check out the ACOT2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACOT2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ACOT2 Antibody (A10261)
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No publications found for A10261
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Customer Q&As
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5 Customer Q&As for Anti-ACOT2 Antibody
Question
Can you help my question with product A10261, anti-ACOT2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10261 anti-ACOT2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-03
Question
Will A10261 anti-ACOT2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
It shows on the product datasheet, A10261 anti-ACOT2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-20
Question
Is this A10261 anti-ACOT2 antibody reactive to the isotypes of ACOT2?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
The immunogen of A10261 anti-ACOT2 antibody is Synthesized peptide derived from internal of human ACOT2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-02
Question
I was wanting to use your anti-ACOT2 antibody for WB for human lung uterus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human lung uterus identification?
Verified Customer
Verified customer
Asked: 2019-04-17
Answer
As indicated on the product datasheet, A10261 anti-ACOT2 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lung uterus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-17
Question
We are currently using anti-ACOT2 antibody A10261 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-03
Answer
The anti-ACOT2 antibody (A10261) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-03