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Product Info Summary
| SKU: | A05688 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Acetyl-eIF5A/eIF5A2 (K47) Antibody
SKU/Catalog Number
A05688
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Acetyl-eIF5A/eIF5A2 (K47) Antibody catalog # A05688. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Acetyl-eIF5A/eIF5A2 (K47) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05688)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized Acetyl-peptide derived from human eIF5A around the Acetylation site of Lys47. AA range:11-60
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A05688 is reactive to EIF5A2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
16793 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05688 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
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Western Blot (WB) analysis of L929 lysis using antibody diluted at 1:1000.
Protein Target Info & Infographic
Gene/Protein Information For EIF5A2 (Source: Uniprot.org, NCBI)
Gene Name
EIF5A2
Full Name
Eukaryotic translation initiation factor 5A-2
Weight
16793 MW
Superfamily
eIF-5A family
Alternative Names
Eukaryotic translation initiation factor 5A-2;eIF-5A-2;eIF-5A2;Eukaryotic initiation factor 5A isoform 2;EIF5A2; EIF5A2 EIF-5A2, eIF5AII eukaryotic translation initiation factor 5A2 eukaryotic translation initiation factor 5A-2|eIF-5A-2|eukaryotic initiation factor 5A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EIF5A2, check out the EIF5A2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EIF5A2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Acetyl-eIF5A/eIF5A2 (K47) Antibody (A05688)
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Customer Q&As
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6 Customer Q&As for Anti-Acetyl-eIF5A/eIF5A2 (K47) Antibody
Question
Is this A05688 anti-Acetyl-eIF5A/eIF5A2 (K47) antibody reactive to the isotypes of EIF5A2?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
The immunogen of A05688 anti-Acetyl-eIF5A/eIF5A2 (K47) antibody is Synthesized peptide derived from human eIF5A/eIF5A2 around the acetylation site of K47. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-14
Question
We are currently using anti-Acetyl-eIF5A/eIF5A2 (K47) antibody A05688 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
The anti-Acetyl-eIF5A/eIF5A2 (K47) antibody (A05688) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-16
Question
Does A05688 anti-Acetyl-eIF5A/eIF5A2 (K47) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-04-25
Answer
It shows on the product datasheet, A05688 anti-Acetyl-eIF5A/eIF5A2 (K47) antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-04-25
Question
Is a blocking peptide available for product anti-Acetyl-eIF5A/eIF5A2 (K47) antibody (A05688)?
M. Li
Verified customer
Asked: 2017-09-04
Answer
We do provide the blocking peptide for product anti-Acetyl-eIF5A/eIF5A2 (K47) antibody (A05688). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-09-04
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Acetyl-eIF5A/eIF5A2 (K47) antibody A05688. Let me know if you need anything else.
N. Zhao
Verified customer
Asked: 2016-10-10
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-10-10
Question
We want to test anti-Acetyl-eIF5A/eIF5A2 (K47) antibody A05688 on human brain for research purposes, then I may be interested in using anti-Acetyl-eIF5A/eIF5A2 (K47) antibody A05688 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Patel
Verified customer
Asked: 2016-05-24
Answer
The products we sell, including anti-Acetyl-eIF5A/eIF5A2 (K47) antibody A05688, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-05-24
