Anti-Acetyl-eIF5A/eIF5A2 (K47) Antibody

Product Name

Anti-Acetyl-eIF5A/eIF5A2 (K47) Antibody

View all EIF5A2 Antibodies

SKU/Catalog Number

A05688

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Acetyl-eIF5A/eIF5A2 (K47) Antibody catalog # A05688. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Acetyl-eIF5A/eIF5A2 (K47) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05688)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human eIF5A/eIF5A2 around the acetylation site of K47.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A05688 is reactive to EIF5A2 in Human, Mouse, Rat

Applications

A05688 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

16793 MW

Background of EIF5A2

ROC2 also known as RING-box protein 2, Rbx2, RING finger protein 7, Regulator of cullins 2, CKII beta-binding protein 1, and CKBBP1, is a probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. ROC2 appears to recruit the E2 ubiquitination enzyme through the RING-type zinc finger in a manner similar to CDC34, and brings it into close proximity to the substrate. ROC2 may play a role in protecting cells from apoptosis induced by redox agents. ROC2 has a cytoplasmic and nuclear localization and is expressed in heart, liver, skeletal muscle and pancreas tissues, and at very low levels in brain, placenta and lung. 1,10-phenanthroline induces ROC2 expression. The RING-type zinc finger domain is essential for ubiquitin ligase activity. Phosphorylation by CK2 is required for efficient degradation of NFKBIA and CDKN1B.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

User needs to optimize the dilution ratio for this antibody.

Validation Images & Assay Conditions

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Western Blot (WB) analysis of L929 lysis using antibody diluted at 1:1000.

Gene/Protein Information For EIF5A2 (Source: Uniprot.org, NCBI)

Alternative Names

EIF-5A2; eIF-5A-2; eIF5AII; Eukaryotic initiation factor 5A isoform 2; eukaryotic initiation factor 5A; eukaryotic translation initiation factor 5A2; eukaryotic translation initiation factor 5A-2 EIF5A2 EIF-5A2, eIF5AII eukaryotic translation initiation factor 5A2 eukaryotic translation initiation factor 5A-2|eIF-5A-2|eukaryotic initiation factor 5A

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In this infographic, you will see the following information for EIF5A2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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