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Product Info Summary
| SKU: | A05368-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Aak1 Antibody
SKU/Catalog Number
A05368-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Aak1 Antibody (Catalog # A05368-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Aak1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Aak1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05368-1)
Host
Rabbit
Contents
Aak1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Aak1 antibody was raised against a 20 amino acid synthetic peptide near the amino terminus of the human Aak1. The immunogen is located within amino acids 190 - 240 of Aak1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Multiple isoforms of GLS2 are known to exist.
Reactive Species
A05368-1 is reactive to AAK1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
103885 MW
Background of AAK1
AP2-associated protein kinase 1 (Aak1) is a member of the Ark1/Prk1 subfamily of Ser/Thr protein kinases that are thought to regulate endocytosis by phosphorylating the accessory endocytic components. Aak1 interacts with and phosphorylates the mu2 subunit of the AP-2 complex, which promotes binding of the AP-2 to tyrosine based (Yxxphi) internalization motif-containing receptors and subsequent receptor endocytosis. At least two isoforms of Aak1 are known to exist; the longer isoform contains an extended carboxy-terminus that contains an additional clathrin-binding domain. Overexpression of this long isoform or Aak1 depletion by RNA interference impairs transferrin recycling from the early/sorting endosome, suggesting that Aak1 functions at multiple steps of the endosomal pathway by regulating transferrin internalization and its recycling back to the plasma membrane.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05368-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Aak1 antibody can be used for detection of Aak1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Aak1 in A-20 lysate with Aak1 antibody at (A) 1 and (B) 2 μg/mL.
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Immunocytochemistry of Aak1 in A-20 cells with Aak1 antibody at 5 μg/mL.
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Immunofluorescence of aak1 in human brain tissue with aak1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For AAK1 (Source: Uniprot.org, NCBI)
Gene Name
AAK1
Full Name
AP2-associated protein kinase 1
Weight
103885 MW
Superfamily
protein kinase superfamily
Alternative Names
KIAA1048, AP2-associated protein kinase 1, Adaptor-associated kinase 1 AAK1 AP2 associated kinase 1 AP2-associated protein kinase 1|adaptor-associated kinase 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AAK1, check out the AAK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AAK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Aak1 Antibody (A05368-1)
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