AAK1 Antibodies

AND AAK1 ELISA kits, AAK1 Proteins

Many biological assays use antibodies to detect AAK1, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with AAK1 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop AAK1 antibodies...

We validate the specificity of these antibodies to AAK1 by testing them on tissues known to express AAK1 positively and negatively. Browse below to find the AAK1 antibody that suites your experiment. We have 4 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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AAK1 Infographic by Boster Bio

All AAK1 Products

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Specificity

Applications

Price

AAK1 Colorimetric Cell-Based ELISA

Human, Mouse, Rat

ELISA

$480
Cat # EKC1842
1 kit, containing one 96-well plate and all necessary reagents

Anti-Aak1 Antibody

Human, Mouse, Rat

ELISA, WB

$449
Cat # A05368
0.1 mg

Anti-Aak1 Antibody

Human, Mouse, Rat

ELISA, IF, ICC, WB

$449
Cat # A05368-1
0.1 mg

Anti-AAK1 Antibody

Human, Monkey, Mouse, Rat

ELISA, IF, IHC, WB

$380
Cat # A05368-2
100ul

AAK1 Overview

Facts about AP2-associated protein kinase 1.

AAK1 3D structure. Source: alphafold

AP2-associated protein kinase 1 (AAK1)

Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to freight membrane proteins during the beginning stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1.

Regulates phosphorylation of other AP-2 subunits in addition to AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, boosting NUMB localization into endosomes.

Gene Information

Human
Gene Name:	AAK1
Uniprot:	Q2M2I8
Entrez:	22848

Family

Belongs to:

protein kinase superfamily

Alternative Names

AAK1; Adaptor-associated kinase 1; AP2 associated kinase 1; AP2-associated protein kinase 1; DKFZp686F03202; DKFZp686K16132; EC 2.7.11.1; FLJ23712; FLJ25931; FLJ31060; FLJ45252; KIAA1048FLJ42882; MGC138170; MGC164568; MGC164570

Molecular Weight

Mass (kDA):
103.885 kDA

Genomic Context


Human

Location:	2p13.3
Sequence:	2; NC_000002.12 (69457997..69643739, complement)

Tissue Specificity

Detected in brain, heart and liver. Isoform 1 is the predominant isoform in brain.

Subcellular Localizations

Cell membrane; Peripheral membrane protein. Membrane, clathrin-coated pit. Cell junction, synapse, presynapse. Active when found in clathrin-coated pits at the plasma membrane. In neuronal cells, enriched at presynaptic terminals. In non-neuronal cells, enriched at leading edge of migrating cells.