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Product Info Summary
| SKU: | A04148-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-14-3-3 gamma/YWHAG Antibody Picoband®
View all 14-3-3 gamma Antibodies
SKU/Catalog Number
A04148-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-14-3-3 gamma/YWHAG Antibody Picoband® catalog # A04148-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-14-3-3 gamma/YWHAG Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04148-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human 14-3-3 gamma/YWHAG recombinant protein (Position: D21-K162).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04148-2 is reactive to YWHAG in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
28 kDa
Calculated molecular weight
211344 MW
Background of 14-3-3 gamma
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04148-2 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Jurkat whole cell, human placenta tissue, rat brain tissue, rat skeletal muscle tissue, mouse brain tissue, mouse skeletal muscle tissue
IHC: human esophageal squamous carcinoma tissue
FCM: U87 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of 14-3-3 gamma/YWHAG using anti-14-3-3 gamma/YWHAG antibody (A04148-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human placenta tissue lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat skeletal muscle tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse skeletal muscle tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-14-3-3 gamma/YWHAG antigen affinity purified polyclonal antibody (Catalog # A04148-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 14-3-3 gamma/YWHAG at approximately 28 kDa. The expected band size for 14-3-3 gamma/YWHAG is at 28 kDa.
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Figure 2. IHC analysis of 14-3-3 gamma/YWHAG using anti-14-3-3 gamma/YWHAG antibody (A04148-2).
14-3-3 gamma/YWHAG was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-14-3-3 gamma/YWHAG Antibody (A04148-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. Flow Cytometry analysis of U87 cells using anti-14-3-3 gamma/YWHAG antibody (A04148-2).
Overlay histogram showing U87 cells stained with A04148-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-14-3-3 gamma/YWHAG Antibody (A04148-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For YWHAG (Source: Uniprot.org, NCBI)
Gene Name
YWHAG
Full Name
14-3-3 protein gamma
Weight
211344 MW
Superfamily
14-3-3 family
Alternative Names
Prostaglandin E synthase 3; Cytosolic prostaglandin E2 synthase; Cpges; Hsp90 co-chaperone; Progesterone receptor complex p23; Telomerase-binding protein p23; PTGES3; P23, TEBP YWHAG 14-3-3GAMMA, DEE56, EIEE56, PPP1R170 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma 14-3-3 protein gamma|KCIP-1|protein kinase C inhibitor protein 1|protein phosphatase 1, regulatory subunit 170|tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on YWHAG, check out the YWHAG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for YWHAG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-14-3-3 gamma/YWHAG Antibody Picoband® (A04148-2)
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