This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
Facts about 14-3-3 protein gamma.
Binding generally results in the modulation of the activity of the binding partner. .
Human | |
---|---|
Gene Name: | YWHAG |
Uniprot: | P61981 |
Entrez: | 7532 |
Belongs to: |
---|
14-3-3 family |
1433 gamma; 14-3-3 gamma; 14-3-3 protein gamma; 14-3-3GAMMA; KCIP-1; Protein kinase C inhibitor protein 1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gammapolypeptide; YWHAG
Mass (kDA):
28.303 kDA
Human | |
---|---|
Location: | 7q11.23 |
Sequence: | 7; NC_000007.14 (76326799..76358991, complement) |
Highly expressed in brain, skeletal muscle, and heart.
Cytoplasm.
YWHAG is a protein that mediates signal transduction. It can be detected using Enhanced Chemiluminescence Detection. To gain product credits, Boster scientists can submit results. This applies to scientists around the world. Boster Bio has many uses of the YWHAG Marker. Here are some examples. Read on to discover how this adaptor protein can be used in your research.
A protein called YWHAG has been identified as an oncogene in the TCGA Database. This gene is a key regulator of cell proliferation and invasion, and YWHAG overexpression correlated with a poorer prognosis in cervical cancer. YWHAG was also statistically significant among cervical cancer patients. It is not known whether this protein is directly to blame for poor prognosis in cervical carcinoma patients.
Researchers discovered that YWHAG, a new member the 14-3-3 proteins family, was discovered by researchers. This family of proteins regulates various signal transduction pathways by binding to phosphoserine-containing proteins. YWHAG plays a key role in signal transduction, as well as the regulation of v.raf-1 Murine Leukemia Viral Oncogene Holog 1, and pro-tein kinase.
The YWHAG Marker - an adapter protein - plays a critical role in brain circuitry development. The genetic mutations have been linked to autism, epilepsy, developmental disorder, and epilepsy. But, it is not known if there are other phenotypes. There is also a correlation between YWHAG-mutations and myoclonic epilepsy. Some cases have mild myoclonic epilepsy due to a mutation in this gene, while others can cause severe and uncontrollable EE.
YWHAG is a member the protein family14-3-3. It regulates multiple cell signaling pathways. Several YWHAG mutations were identified in epileptic encephalopathy patients. Two novel YWHAG mutations were discovered in two unrelated families. Both of these mutations involved the heterozygous truncating mutation (c.124C>T/p.Arg42Ter) and a de novo missense mutation. The probands in both families suffered daily myoclonic seizures. Both probands were able to have seizures-free episodes with valproate therapy. Another affected family member presented with febrile seizures.
Enhanced chemiluminescence (also known as Western chemiluminescence) is a popular technique for protein detection. Its inherent sensitivity and high signal–to-noise ratio make it a viable technique for the detection of many biological materials. This article will explain how YWHAG can detected using ECL.
ECL is based primarily on HP-conjugated antibodies. The target enzyme is present in the antibody, and the antibodies react with it to release excited intermediates. These intermediates emit strong blue emission at 450nm. The enzyme-substrate reacts only with the antibodies, and the light emission ceases once the substrate is exhausted.
Enhanced Chemiluminescence (EC) - This method uses two types chemiluminescent substrates, horseradish peroxidase or dioxetanephenolate. The latter is suitable for western blot applications containing abundant proteins. These two substrates are also suited for optimized experiments.
ECL is extremely sensitive - it can detect picogram and femtogram amounts with one reaction. Chemiluminescent identification can detect proteins in very low concentrations. This technique is especially useful in protein expression and purification. Luminol's half-life is relatively short. An enhanced chemiluminescent substrat extends this time to 24 hours. ECL allows for reimaging with no degradation of signal intensity.
Enhanced Chemiluminescence – Using a reagent containing p–coumaric acids and luminol to detect YWHAG, ECL can be used. ECL allows researchers the ability to compare YWHAG concentrations using a variety of biological samples including metabolites, proteins, hormones, and more.
Enhanced Chemiluminescence - ECL allows researchers to detect YWHAG in samples that have undergone protein purification. The HA tagged positive control lysate has a molecularweight marker for nucleic substances. The enzyme was incubated with blocking buffer for 60 minutes.
PMID: 10433554 by Autieri M.V., et al. 14-3-3gamma interacts with and is phosphorylated by multiple protein kinase C isoforms in PDGF-stimulated human vascular smooth muscle cells.
PMID: 10486217 by Horie M., et al. Cloning, expression, and chromosomal mapping of the human 14-3-3gamma gene (YWHAG) to 7q11.23.