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Product Info Summary
| SKU: | EKC2642 |
|---|---|
| Size: | 1 kit, containing two 96-well plates and all necessary reagents |
| Reactive Species: | Human, Mouse |
| Application: | ELISA |
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Product info
Product Name
Abl (Phospho-Tyr393/412) Colorimetric Cell-Based ELISA
SKU/Catalog Number
EKC2642
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The Abl (Phospho-Tyr393/412) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Abl (Phospho-Tyr393/412) protein expression profile in cells. The kit can be used for measuring the relative amounts of Abl (Phospho-Tyr393/412) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Abl (Phospho-Tyr393/412).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
Abl (Phospho-Tyr393/412) Colorimetric Cell-Based ELISA (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2642)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC2642 is reactive to ABL1 in Human, Mouse samples
Application Guarantee
EKC2642 is guaranteed for ELISA in Human, Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 2 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-Abl Phospho-Tyr393/412 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Red |
| 100x Anti-Abl Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 12ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 12ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 12ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of lysates from RAW264.7 cells
Protein Target Info & Infographic
Gene/Protein Information For ABL1 (Source: Uniprot.Org, NCBI)
Gene Name
ABL1
Full Name
Tyrosine-protein kinase ABL1
Weight
122873 MW
Superfamily
protein kinase superfamily
Alternative Names
Tyrosine-protein kinase ABL1;2.7.10.2 ;Abelson murine leukemia viral oncogene homolog 1;Abelson tyrosine-protein kinase 1;Proto-oncogene c-Abl;p150;ABL1;ABL, JTK7; ABL1 ABL, BCR-ABL, CHDSKM, JTK7, bcr/abl, c-ABL, c-ABL1, p150, v-abl ABL proto-oncogene 1, non-receptor tyrosine kinase tyrosine-protein kinase ABL1|ABL protooncogene 1 nonreceptor tyrosine kinase|Abelson tyrosine-protein kinase 1|bcr/c-abl oncogene protein|c-abl oncogene 1, receptor tyrosine kinase|proto-oncogene c-Abl|proto-oncogene tyrosine-protein kinase ABL1|truncated ABL protooncogene 1 nonreceptor tyrosine kinase|v-abl Abelson murine leukemia viral oncogene homolog 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ABL1, check out the ABL1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ABL1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Abl (Phospho-Tyr393/412) Colorimetric Cell-Based ELISA (EKC2642)
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