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Product Info Summary
| SKU: | PB9988 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Bcr Antibody Picoband®
SKU/Catalog Number
PB9988
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Bcr Antibody Picoband® catalog # PB9988. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Bcr Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9988)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Bcr recombinant protein (Position: M1-A100). Human Bcr shares 94% amino acid (aa) sequence identity with mouse Bcr.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9988 is reactive to BCR in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
143 kDa
Calculated molecular weight
142819 MW
Background of BCR
The breakpoint cluster region protein (BCR) is a protein that in humans is encoded by the BCR gene. A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9988 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human K562 whole cell, human 293T whole cell, human HepG2 whole cell, rat brain tissue, mouse brain tissue
ICC/IF: A431 cell
FCM: HL-60 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Bcr using anti-Bcr antibody (PB9988).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bcr antigen affinity purified polyclonal antibody (Catalog # PB9988) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bcr at approximately 143 kDa. The expected band size for Bcr is at 143 kDa.
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Figure 2. IF analysis of Bcr using anti-Bcr antibody (PB9988).
Bcr was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Bcr Antibody (PB9988) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of HL-60 cells using anti-Bcr antibody (PB9988).
Overlay histogram showing HL-60 cells stained with PB9988 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Bcr Antibody (PB9988, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For BCR (Source: Uniprot.org, NCBI)
Gene Name
BCR
Full Name
Breakpoint cluster region protein
Weight
142819 MW
Alternative Names
Breakpoint cluster region protein;2.7.11.1;Renal carcinoma antigen NY-REN-26;BCR;BCR1, D22S11; BCR ALL1, CML, D22S11, D22S662, PHL, BCR BCR activator of RhoGEF and GTPase breakpoint cluster region protein|BCR, RhoGEF and GTPase activating protein|BCR/FGFR1 chimera protein|FGFR1/BCR chimera protein|breakpoint cluster region|renal carcinoma NY-REN-26
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BCR, check out the BCR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BCR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Bcr Antibody Picoband® (PB9988)
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No publications found for PB9988
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Customer Q&As
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15 Customer Q&As for Anti-Bcr Antibody Picoband®
Question
We are currently using anti-Bcr antibody PB9988 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
The anti-Bcr antibody (PB9988) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-06
Question
Is a blocking peptide available for product anti-Bcr antibody (PB9988)?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
We do provide the blocking peptide for product anti-Bcr antibody (PB9988). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-23
Question
Please see the WB image, lot number and protocol we used for leukemic t-cell using anti-Bcr antibody PB9988. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-21
Question
Can you help my question with product PB9988, anti-Bcr antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Roberts
Verified customer
Asked: 2020-02-19
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9988 anti-Bcr antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-19
Question
Do you have a BSA free version of anti-Bcr antibody PB9988 available?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Bcr antibody PB9988 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-31
Question
My colleagues were satisfied with the WB result of your anti-Bcr antibody. However we have been able to see positive staining in cervix carcinoma cell junction using this antibody. Is that expected? Could you tell me where is BCR supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
According to literature, cervix carcinoma does express BCR. Generally BCR expresses in cell junction, synapse, postsynapse,. Regarding which tissues have BCR expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2020-01-17
Question
We want to test anti-Bcr antibody PB9988 on mouse leukemic t-cell for research purposes, then I may be interested in using anti-Bcr antibody PB9988 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
The products we sell, including anti-Bcr antibody PB9988, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-19
Question
Will anti-Bcr antibody PB9988 work for WB with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
According to the expression profile of leukemic t-cell, BCR is highly expressed in leukemic t-cell. So, it is likely that anti-Bcr antibody PB9988 will work for WB with leukemic t-cell.
Boster Scientific Support
Answered: 2019-08-22
Question
Would PB9988 anti-Bcr antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
You can see on the product datasheet, PB9988 anti-Bcr antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-22
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-Bcr antibody PB9988. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-02-20
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-20
Question
I was wanting to use your anti-Bcr antibody for WB for mouse leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse leukemic t-cell identification?
C. Krishna
Verified customer
Asked: 2018-11-20
Answer
As indicated on the product datasheet, PB9988 anti-Bcr antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-20
Question
We have seen staining in rat leukemic t-cell. Are there any suggestions? Is anti-Bcr antibody supposed to stain leukemic t-cell positively?
A. Carter
Verified customer
Asked: 2017-10-04
Answer
From literature leukemic t-cell does express BCR. From Uniprot.org, BCR is expressed in adenohypophysis, brain, renal cell carcinoma, leukemic t-cell, cervix carcinoma, platelet, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have BCR expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2017-10-04
Question
Is this PB9988 anti-Bcr antibody reactive to the isotypes of BCR?
J. Moore
Verified customer
Asked: 2017-08-16
Answer
The immunogen of PB9988 anti-Bcr antibody is E.coli-derived human Bcr recombinant protein (Position: M1-A100). Human Bcr shares 94% amino acid (aa) sequence identity with mouse Bcr. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-08-16
Question
I was wanting to use using your anti-Bcr antibody for signaling by fgfr1 in disease studies. Has this antibody been tested with western blotting on testis tissue? We would like to see some validation images before ordering.
D. Li
Verified customer
Asked: 2016-03-07
Answer
I appreciate your inquiry. This PB9988 anti-Bcr antibody is tested on human jurkat whole cell lysates, rat brain tissue, mouse brain, testis tissue. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-03-07
Question
I see that the anti-Bcr antibody PB9988 works with WB, what is the protocol used to produce the result images on the product page?
G. Brown
Verified customer
Asked: 2016-01-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-01-22
