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Product Info Summary
| SKU: | PA1808 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-Crk-like protein CrkL Antibody Picoband®
SKU/Catalog Number
PA1808
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Crk-like protein CrkL Antibody catalog # PA1808. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Crk-like protein CrkL Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1808)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CrkL, different from the related mouse and rat sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1808 is reactive to CRKL in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
39 kDa
Calculated molecular weight
33777 MW
Background of CRKL
CRKL (v-crk sarcoma virus CT10 oncogene homolog (avian)-like) also called ONCOGENE CRKL, encodes a 303-amino acid polypeptide with a predicted molecular mass of 36 kD. While CRKL is not the human homolog of CRK, it is similar to protein-tyrosine kinases with SH2 and SH3 (src homology) domains. By fluorescence in situ hybridization, the CRKL gene is localized to chromosome 22q11, centromeric of the chronic myelogenous leukemia breakpoint region. Senechal et al. (1996) showed that CRKL becomes phosphorylated when overexpressed, activates Ras-dependent and JNK pathways, and transforms fibroblasts. The authors also found CRKL to be a substrate for the BCR-ABL tyrosine kinase, leading them to conclude thatCRKL is a tyrosine kinase and an oncogene. Crkl was required for normal cellular responses to Fgf8, including survival and migration, Erk activation, and target gene expression.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1808 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human K562 whole cell, human 293T whole cell, human U20S whole cell, human Hela whole cell, rat thymus tissue, rat brain tissue, mouse thymus tissue, mouse brain tissue
FCM: U251 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CRKL using anti-CRKL antibody (PA1808).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human U20S whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: rat thymus tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse thymus tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRKL antigen affinity purified polyclonal antibody (Catalog # PA1808) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRKL at approximately 39 kDa. The expected band size for CRKL is at 34 kDa.
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Figure 2. Flow Cytometry analysis of U251 cells using anti-CRKL antibody (PA1808).
Overlay histogram showing U251 cells stained with PA1808 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CRKL Antibody (PA1808, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For CRKL (Source: Uniprot.org, NCBI)
Gene Name
CRKL
Full Name
Crk-like protein
Weight
33777 MW
Superfamily
CRK family
Alternative Names
Crk-like protein;CRKL; CRKL CRK like proto-oncogene, adaptor protein crk-like protein|v-crk avian sarcoma virus CT10 oncogene homolog-like
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CRKL, check out the CRKL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CRKL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Crk-like protein CrkL Antibody Picoband® (PA1808)
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No publications found for PA1808
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Customer Q&As
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5 Customer Q&As for Anti-Crk-like protein CrkL Antibody Picoband®
Question
Is there a BSA free version of anti-CrkL antibody PA1808 available?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CrkL antibody PA1808 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-28
Question
Is a blocking peptide available for product anti-CrkL antibody (PA1808)?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
We do provide the blocking peptide for product anti-CrkL antibody (PA1808). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-24
Question
I see that the anti-CrkL antibody PA1808 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-05
Question
We are currently using anti-CrkL antibody PA1808 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2018-05-29
Answer
The anti-CrkL antibody (PA1808) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-29
Question
See attached the WB image, lot number and protocol we used for secondary oocyte using anti-CrkL antibody PA1808. Please let me know if you require anything else.
J. Krishna
Verified customer
Asked: 2015-07-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-07-10
