Anti-IDO2 Monoclonal Antibody

Product Name

Anti-IDO2 Monoclonal Antibody

View all IDO2 Antibodies

SKU/Catalog Number

M06002

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-IDO2 Monoclonal Antibody (Catalog # M06002). Tested in WB applications. This antibody reacts with Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-IDO2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06002)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: 1HC

Isotype

IgG2a kappa

Immunogen

Anti-IDO2 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with complete mouse IDO2 protein followed by hybridoma development.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~550kDa. No cross-reactivity to Bassoon.

Reactive Species

M06002 is reactive to IDO2 in Mouse

Applications

M06002 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

30063 MW

Background of IDO2

Indoleamine 2,3-dioxygenase 2 (IDO2) is related to IDO1, and may play a role in T and B Cell Immunity. IDO2’s role in immune regulation will be become clearer following the completion of genetic knock-out studies. IDO2 is expressed in a few select cell types and levels of protein expression appear to be very low, perhaps below the level of detection by IHC. Small-molecule inhibitors of indoleamine 2,3-dioxygenase (IDO) are currently being translated to clinic for evaluation as cancer therapeutics.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5000 - 1:50000
IF Microscopy: 1:50-1:100
WB: 1:500-1:1500

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Ido2 using anti-Ido2 antibody (M06002).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ido2 antigen affinity purified polyclonal antibody (Catalog # M06002) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for Ido2.

Gene/Protein Information For IDO2 (Source: Uniprot.org, NCBI)

Alternative Names

EC 1.13.11.-; IDO2; Ido-2; INDOL1; INDOL1indoleamine-pyrrole 2,3 dioxygenase-like 1; indoleamine 2,3-dioxygenase 2; Indoleamine 2,3-dioxygenase-2; indoleamine 2,3-dioxygenase-like 1 protein; Indoleamine 2,3-dioxygenase-like protein 1; Indoleamine-pyrrole 2,3-dioxygenase-like protein 1 IDO2 INDOL1 indoleamine 2,3-dioxygenase 2 indoleamine 2,3-dioxygenase 2|indoleamine 2,3-dioxygenase-like 1 protein|indoleamine 2,3-dioxygenase-like protein 1|indoleamine-pyrrole 2,3 dioxygenase-like 1|indoleamine-pyrrole 2,3-dioxygenase-like protein 1

For more info on IDO2, check out the IDO2 Infographic

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In this infographic, you will see the following information for IDO2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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