Product Info Summary
SKU: | LS007311 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of ubiquitin A-52 residue ribosomal protein fusion product 1 (UBA52), transcript variant 1 |
Customers Who Bought This Also Bought
Product info
Product Name
UBA52 (NM_001033930) Human Over-expression Lysate
SKU/Catalog Number
LS007311
Size
100 µg
Description
Transient overexpression lysate of ubiquitin A-52 residue ribosomal protein fusion product 1 (UBA52), transcript variant 1
Storage & Handling
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Cite This Product
UBA52 (NM_001033930) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS007311)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
![ls007311 uba52 human over expression lysates nm 001033930 wb testing 1 ls007311 uba52 human over expression lysates nm 001033930 wb testing 1](https://www.bosterbio.com/media/catalog/product/l/s/ls007311-uba52-human-over-expression-lysates-nm-001033930-wb-testing-1.jpg)
Click image to see more details
UBA52 Human Over-expression Lysates NM_001033930.
Western blot validation of overexpression lysate (LS007311) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with UBA52 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
![ls007311 uba52 human over expression lysates nm 003333 wb testing 2 ls007311 uba52 human over expression lysates nm 003333 wb testing 2](https://www.bosterbio.com/media/catalog/product/l/s/ls007311-uba52-human-over-expression-lysates-nm-003333-wb-testing-2.jpg)
Click image to see more details
UBA52 Human Over-expression Lysates NM_003333.
Western blot validation of overexpression lysate (LS007311) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with UBA52 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for UBA52 (Source: Uniprot.org, NCBI)
Gene Name
UBA52
Full Name
Ubiquitin-60S ribosomal protein L40
Weight
Alternative Names
CEP52ubiquitin carboxyl extension protein 52; HUBCEP52; MGC126879; MGC126881; MGC57125; RPL40; UBCEP2; ubiquitin A-52 residue ribosomal protein fusion product 160S ribosomal protein L40; ubiquitin-52 amino acid fusion protein; ubiquitin-60S ribosomal protein L40; ubiquitin-CEP52 UBA52 CEP52, HUBCEP52, L40, RPL40 ubiquitin A-52 residue ribosomal protein fusion product 1 ubiquitin-60S ribosomal protein L40|ubiquitin carboxyl extension protein 52|ubiquitin-52 amino acid fusion protein|ubiquitin-CEP52
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on UBA52, check out the UBA52 Infographic
![UBA52 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UBA52: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For UBA52 (NM_001033930) Human Over-expression Lysate (LS007311)
Hello CJ!
No publications found for LS007311
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful:
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used UBA52 (NM_001033930) Human Over-expression Lysate?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For UBA52 (NM_001033930) Human Over-expression Lysate
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question