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Product Info Summary
| SKU: | EKC1658 |
|---|---|
| Size: | 1 kit, containing one 96-well plate and all necessary reagents |
| Reactive Species: | Human |
| Application: | ELISA |
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Product info
Product Name
TNFSF15 Colorimetric Cell-Based ELISA
SKU/Catalog Number
EKC1658
Size
1 kit, containing one 96-well plate and all necessary reagents
Description
The TNFSF15 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor TNFSF15 protein expression profile in cells. The kit can be used for measuring the relative amounts of TNFSF15 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on TNFSF15.
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
TNFSF15 Colorimetric Cell-Based ELISA (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1658)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1658 is reactive to TNFSF15 in Human samples
Application Guarantee
EKC1658 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 1 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-TNFSF15 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 6ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 6ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 6ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from COLO205 cells
Protein Target Info & Infographic
Gene/Protein Information For TNFSF15 (Source: Uniprot.Org, NCBI)
Gene Name
TNFSF15
Full Name
Tumor necrosis factor ligand superfamily member 15
Weight
28087 MW
Superfamily
tumor necrosis factor family
Alternative Names
Tumor necrosis factor ligand superfamily member 15;TNF ligand-related molecule 1;Vascular endothelial cell growth inhibitor;Tumor necrosis factor ligand superfamily member 15, membrane form;Tumor necrosis factor ligand superfamily member 15, secreted form;TNFSF15;TL1, VEGI; TNFSF15 TL1, TL1A, TNLG1B, VEGI, VEGI192A TNF superfamily member 15 tumor necrosis factor ligand superfamily member 15|TNF ligand-related molecule 1|TNF superfamily ligand TL1A|tumor necrosis factor (ligand) superfamily, member 15|tumor necrosis factor ligand 1B|tumor necrosis factor superfamily member 15|vascular endothelial cell growth inhibitor|vascular endothelial growth inhibitor-192A
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TNFSF15, check out the TNFSF15 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TNFSF15: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For TNFSF15 Colorimetric Cell-Based ELISA (EKC1658)
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