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Product Info Summary
| SKU: | EKC1805 |
|---|---|
| Size: | 1 kit, containing one 96-well plate and all necessary reagents |
| Reactive Species: | Human, Mouse, Rat |
| Application: | ELISA |
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Product info
Product Name
TF2H2 Colorimetric Cell-Based ELISA
SKU/Catalog Number
EKC1805
Size
1 kit, containing one 96-well plate and all necessary reagents
Description
The TF2H2 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor TF2H2 protein expression profile in cells. The kit can be used for measuring the relative amounts of TF2H2 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on TF2H2.
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
TF2H2 Colorimetric Cell-Based ELISA (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1805)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1805 is reactive to GTF2H2 in Human, Mouse, Rat samples
Application Guarantee
EKC1805 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 1 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-TF2H2 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 6ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 6ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 6ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from COLO205 cells
Protein Target Info & Infographic
Gene/Protein Information For GTF2H2 (Source: Uniprot.Org, NCBI)
Gene Name
GTF2H2
Full Name
General transcription factor IIH subunit 2
Weight
44419 MW
Superfamily
GTF2H2 family
Alternative Names
General transcription factor IIH subunit 2;Basic transcription factor 2 44 kDa subunit;BTF2 p44 ;General transcription factor IIH polypeptide 2;TFIIH basal transcription factor complex p44 subunit ;GTF2H2;BTF2P44; GTF2H2 BTF2, BTF2P44, T-BTF2P44, TFIIH, p44 general transcription factor IIH subunit 2 general transcription factor IIH subunit 2|BTF2 p44|TFIIH basal transcription factor complex p44 subunit|basic transcription factor 2 44 kDa subunit|general transcription factor IIH polypeptide 2|general transcription factor IIH, polypeptide 2, 44kD subunit|general transcription factor IIH, polypeptide 2, 44kDa
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on GTF2H2, check out the GTF2H2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for GTF2H2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For TF2H2 Colorimetric Cell-Based ELISA (EKC1805)
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