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Product Info Summary
| SKU: | A01580-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Rad52 Antibody
SKU/Catalog Number
A01580-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Rad52 Antibody catalog # A01580-1. Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rad52 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01580-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human RAD52. AA range:70-119
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01580-1 is reactive to RAD52 in Human, Mouse
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
46169 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01580-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of 293 cells using Rad52 Polyclonal antibody.
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Western Blot (WB) analysis of specific cells using Rad52 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For RAD52 (Source: Uniprot.org, NCBI)
Gene Name
RAD52
Full Name
DNA repair protein RAD52 homolog
Weight
46169 MW
Superfamily
RAD52 family
Alternative Names
DNA repair protein RAD52 homolog;RAD52; RAD52 RAD52 homolog, DNA repair protein DNA repair protein RAD52 homolog|recombination protein RAD52|rhabdomyosarcoma MU-RMS-40.23
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAD52, check out the RAD52 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAD52: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rad52 Antibody (A01580-1)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Rad52 Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-Rad52 antibody A01580-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-05-01
Question
Is there a BSA free version of anti-Rad52 antibody A01580-1 available?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Rad52 antibody A01580-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-26
Question
Will anti-Rad52 antibody A01580-1 work on pig WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
Our lab technicians have not tested anti-Rad52 antibody A01580-1 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Rad52 antibody A01580-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-29
Question
Will A01580-1 anti-Rad52 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
As indicated on the product datasheet, A01580-1 anti-Rad52 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-25
Question
I would like to test anti-Rad52 antibody A01580-1 on human testis for research purposes, then I may be interested in using anti-Rad52 antibody A01580-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Patel
Verified customer
Asked: 2019-06-10
Answer
The products we sell, including anti-Rad52 antibody A01580-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-10
Question
I was wanting to use your anti-Rad52 antibody for WB for human testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human testis identification?
C. Anderson
Verified customer
Asked: 2018-11-16
Answer
As indicated on the product datasheet, A01580-1 anti-Rad52 antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-16
Question
We are currently using anti-Rad52 antibody A01580-1 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on monkey tissues as well?
R. Johnson
Verified customer
Asked: 2014-04-29
Answer
The anti-Rad52 antibody (A01580-1) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-04-29
