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Product Info Summary
| SKU: | EKC2534 |
|---|---|
| Size: | 1 kit, containing two 96-well plates and all necessary reagents |
| Reactive Species: | Human |
| Application: | ELISA |
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Product info
Product Name
Tau (Phospho-Thr181) Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC2534
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The Tau (Phospho-Thr181) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Tau (Phospho-Thr181) protein expression profile in cells. The kit can be used for measuring the relative amounts of Tau (Phospho-Thr181) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Tau (Phospho-Thr181).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
Tau (Phospho-Thr181) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2534)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC2534 is reactive to MAPT in Human samples
Application Guarantee
EKC2534 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 2 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-Tau Phospho-Thr181 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Red |
| 100x Anti-Tau Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 12ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 12ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 12ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from mouse brain
Protein Target Info & Infographic
Gene/Protein Information For MAPT (Source: Uniprot.Org, NCBI)
Gene Name
MAPT
Full Name
Microtubule-associated protein tau
Weight
78928 MW
Alternative Names
Microtubule-associated protein tau;Neurofibrillary tangle protein;Paired helical filament-tau;PHF-tau;MAPT;MAPTL, MTBT1, TAU; MAPT DDPAC, FTDP-17L, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, tau-40, MAPT microtubule associated protein tau microtubule-associated protein tau|G protein beta1/gamma2 subunit-interacting factor 1|PHF-tau|neurofibrillary tangle protein|paired helical filament-tau|protein phosphatase 1, regulatory subunit 103
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MAPT, check out the MAPT Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MAPT: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Tau (Phospho-Thr181) Colorimetric Cell-Based ELISA Kit (EKC2534)
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Customer Q&As
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1 Customer Q&As for Tau (Phospho-Thr181) Colorimetric Cell-Based ELISA Kit
Question
Are EKC2534 and EKC1559 suitable for Human Serum samples?
Verified customer
Asked: 2019-06-30
Answer
Cell-Based Kits are not intended to be used on serum samples.
Boster Scientific Support
Answered: 2019-06-30
