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Product Info Summary
| SKU: | LS006664 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of SRY (sex determining region Y)-box 11 (SOX11) |
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Product info
Product Name
SOX11 (NM_003108) Human Over-expression Lysate
SKU/Catalog Number
LS006664
Size
100 µg
Description
Transient overexpression lysate of SRY (sex determining region Y)-box 11 (SOX11)
Storage & Handling
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Cite This Product
SOX11 (NM_003108) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006664)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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SOX11 Human Over-expression Lysates NM_003108.
Western blot validation of overexpression lysate (LS006664) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SOX11 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for SOX11 (Source: Uniprot.org, NCBI)
Gene Name
SOX11
Full Name
Transcription factor SOX-11
Weight
46.679kDa
Alternative Names
SOX11; SRY (sex determining region Y)-box 11; SRY (sex-determining region Y)-box 11; SRY-related HMG-box gene 11; transcription factor SOX-11 SOX11 CSS9, MRD27 SRY-box transcription factor 11 transcription factor SOX-11|SRY (sex-determining region Y)-box 11|SRY-box 11|SRY-related HMG-box gene 11
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on SOX11, check out the SOX11 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SOX11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For SOX11 (NM_003108) Human Over-expression Lysate (LS006664)
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