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Product Info Summary
| SKU: | LS009604 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of ring finger protein 14 (RNF14), transcript variant 4 |
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Product info
Product Name
RNF14 (NM_183400) Human Over-expression Lysate
SKU/Catalog Number
LS009604
Size
100 µg
Description
Transient overexpression lysate of ring finger protein 14 (RNF14), transcript variant 4
Storage & Handling
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Cite This Product
RNF14 (NM_183400) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS009604)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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RNF14 Human Over-expression Lysates NM_183400.
Western blot validation of overexpression lysate (LS009604) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RNF14 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for RNF14 (Source: Uniprot.org, NCBI)
Gene Name
RNF14
Full Name
E3 ubiquitin-protein ligase RNF14
Weight
53.837kDa
Superfamily
RBR family
Alternative Names
Androgen receptor-associated protein 54; ARA54; ARA54androgen receptor associated protein 54; EC 6.3.2.-; HFB30; HFB30E3 ubiquitin-protein ligase RNF14; ring finger protein 14FLJ26004; RNF14; Triad2 protein; TRIAD2 RNF14 ARA54, HFB30, HRIHFB2038, TRIAD2 ring finger protein 14 E3 ubiquitin-protein ligase RNF14|androgen receptor associated protein 54
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on RNF14, check out the RNF14 Infographic
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In this infographic, you will see the following information for RNF14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For RNF14 (NM_183400) Human Over-expression Lysate (LS009604)
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