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Product Info Summary
| SKU: | EK0981-EQ |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Equine |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Horse equine TGF-Beta 2 ELISA Kit PicoKine®
View all TGF-beta 2 ELISA kits
SKU/Catalog Number
EK0981-EQ
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Horse equine TGF-Beta 2 ELISA Kit PicoKine® (96 Tests). Quantitate Horse TGFB2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Horse equine TGF-Beta 2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0981-EQ)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: A303-S414
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0981-EQ for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0981-EQ is reactive to TGFB2 in Equine samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK0981-EQ is guaranteed for ELISA in Equine by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TGF-beta 2
Transforming growth factor-beta 2 (TGF-beta 2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. This gene is mapped to 1q41. It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. TGF-beta 2 is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma (POAG). Studies have shown that TGF-beta 2 influences cultured trabecular meshwork cells, and it reduced outflow facility when perfused into cultured horse equine anterior segments. In POAG, elevated expression of Gremlin by TM cells inhibited BMP4 antagonism of TGF-beta 2 and led to increased extracellular matrix deposition and elevated IOP.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0981-EQ-CAP | Anti-Equine TGFB2 Pre-coated 96-well strip microplate | 1 |
| EK0981-EQ-ST | Equine TGFB2 Standard | 2 vials, 10 ng/tube |
| EK0981-EQ-DA | Equine TGFB2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.25 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | |
| O.D. | 0.031 | 0.094 | 0.157 | 0.345 | 0.487 | 1.032 | 1.901 | 2.369 |
Data Example Images
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Horse equine TGF-beta 2 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Horse equine TGF-Beta 2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 221 | 564 | 1145 | 236 | 576 | 1095 |
| Standard deviation | 13.7 | 31.6 | 46.9 | 15.8 | 33.41 | 70.1 |
| CV (%) | 6.2 | 5.6 | 4.1 | 6.7 | 5.8 | 6.4 |
Protein Target Info & Infographic
Gene/Protein Information For TGFB2 (Source: Uniprot.Org, NCBI)
Gene Name
TGFB2
Full Name
Transforming growth factor beta-2 proprotein
Weight
47748 MW
Superfamily
TGF-beta family
Alternative Names
Transforming growth factor beta-2;TGF-beta-2;BSC-1 cell growth inhibitor;Cetermin;Glioblastoma-derived T-cell suppressor factor;G-TSF;Polyergin;Latency-associated peptide;LAP;TGFB2; TGFB2 G-TSF, LDS4, TGF-beta2 transforming growth factor beta 2 transforming growth factor beta-2 proprotein|BSC-1 cell growth inhibitor|cetermin|glioblastoma-derived T-cell suppressor factor|polyergin|prepro-transforming growth factor beta-2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TGFB2, check out the TGFB2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TGFB2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Horse equine TGF-Beta 2 ELISA Kit PicoKine® (EK0981-EQ)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Horse equine TGF-Beta 2 ELISA Kit PicoKine®
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis TGF-beta 2 using the EK0981-EQ Horse TGF-beta 2 Picokine® ELISA Kit?
B. Davis
Verified customer
Asked: 2020-08-18
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2020-08-18
Question
Q: Can TGF-beta 2 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
S. Wu
Verified customer
Asked: 2020-03-05
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-03-05
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-09-08
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-09-08
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-04-06
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-04-06
Question
Q: if the enzyme conjugated TGF-beta 2 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TGF-beta 2 antigen?
Verified Customer
Verified customer
Asked: 2018-12-29
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-12-29
