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Product Info Summary
| SKU: | DZ01398-1 |
|---|---|
| Size: | 200 μl/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
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Product info
Product Name
Anti-Zebrafish Mef2 Antibody
SKU/Catalog Number
DZ01398-1
Size
200 μl/vial
Form
Liquid
Description
Boster Bio Polyclonal Anti-Mef2 Antibody catalog # DZ01398-1. This antibody reacts with Zebrafish.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Zebrafish Mef2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # DZ01398-1)
Host
Rabbit
Contents
Each vial contains 20mM PBS, 50% glycerol, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
DZ01398-1 is reactive to in Zebrafish
Applications
DZ01398-1 is guaranteed for Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Validation Images & Assay Conditions
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Mef2a was detected in a cryosection of zebrafish heart using the rabbit anti-Mef2a antibody (cat# DZ01398-1, 1:200, 4°C overnight). AlexaFluo546 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:200 dilution and incubated for 1.5 h at room temperature.
Specific Publications For Anti-Zebrafish Mef2 Antibody (DZ01398-1)
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Customer Reviews
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1 Reviews For Anti-Zebrafish Mef2 Antibody
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Good Zebrafish Mef2 Antibody from Boster Bio--Jingli Cao, Assistant Professor, Weill Cornell Medical College
Excellent
| Application | Immunofluorescence |
|---|---|
| Sample | Zebrafish heart |
| Detection | Confocal Microscopy |
"Mef2a was detected in a cryosection of zebrafish heart using the rabbit anti-Mef2a antibody (cat# DZ01398-1, 1:200, 4 degrees overnight). AlexaFluor546 Conjugated Goat Anti-Rabbit IgG was used as a secondary antibody at 1:200 dilution and incubated for 1.5 h at room temperature."
Jingli Cao
Verified customer
Submitted 2021-04-13
Customer Q&As
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1 Customer Q&As for Anti-Zebrafish Mef2 Antibody
Question
The immunostaining was done in cryosections using DZ01398-1. Have you ever tried in paraffin sections? Does it work also well in paraffin sections? Does it need any kind of amplification? Tyramide or Biotin-Streptavidin in cryosections or in paraffin sections?
Verified customer
Asked: 2022-03-04
Answer
Our lab hasn't tested the Anti-Zebrafish Mef2 Antibody (DZ01398-1) in paraffin sections. We suggest running a pilot test to see if it could work for the specific paraffin sections. You can use SV or SABC or fluorochrome conjugated secondary antibody. https://www.bosterbio.com/hrp-conjugated-anti-rabbit-igg-super-vision-assay-kit-sv0002-1-boster.html https://www.bosterbio.com/hrp-conjugated-anti-rabbit-igg-sabc-kit-sa1022-boster.html
Boster Scientific Support
Answered: 2022-03-07
