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1 vial of 100 ug empty vector transfected control cell lysate in RIPA buffer
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Varient IDVarient NameDatabase Link
NM_002659transcript variant 1NCBI
NM_001005376transcript variant 2NCBI
NM_001005377transcript variant 3NCBI

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PLAUR (NM_002659) Human Over-expression Lysate

Lysate of uPAR over-expression cell lines, WB control

Transient overexpression lysate of plasminogen activator, urokinase receptor (PLAUR), transcript variant 1

Product Info Summary

SKU: LS005329
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of plasminogen activator, urokinase receptor (PLAUR), transcript variant 1

Product Name

PLAUR (NM_002659) Human Over-expression Lysate

See all uPAR products

SKU/Catalog Number

LS005329

Size

100 µg

Description

Transient overexpression lysate of plasminogen activator, urokinase receptor (PLAUR), transcript variant 1

Storage & Handling

The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.

Cite This Product

PLAUR (NM_002659) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005329)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for PLAUR (Source: Uniprot.org, NCBI)

Gene Name

PLAUR

Full Name

Urokinase plasminogen activator surface receptor

Weight

36.978kDa

Alternative Names

CD87 antigen; CD87; Monocyte activation antigen Mo3; plasminogen activator, urokinase receptor; PLAUR; uPAR; U-PAR; UPARurokinase plasminogen activator surface receptor; u-plasminogen activator receptor form 2; URKRMO3 PLAUR CD87, U-PAR, UPAR, URKR plasminogen activator, urokinase receptor urokinase plasminogen activator surface receptor|monocyte activation Mo3|u-plasminogen activator receptor form 2|urokinase-type plasminogen activator (uPA) receptor

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on PLAUR, check out the PLAUR Infographic

PLAUR infographic

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In this infographic, you will see the following information for PLAUR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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