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Product Info Summary
| SKU: | LS005329 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of plasminogen activator, urokinase receptor (PLAUR), transcript variant 1 |
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Product info
Product Name
PLAUR (NM_002659) Human Over-expression Lysate
SKU/Catalog Number
LS005329
Size
100 µg
Description
Transient overexpression lysate of plasminogen activator, urokinase receptor (PLAUR), transcript variant 1
Storage & Handling
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Cite This Product
PLAUR (NM_002659) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005329)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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PLAUR Human Over-expression Lysates NM_001005376.
Western blot validation of overexpression lysate (LS005329) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PLAUR (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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PLAUR Human Over-expression Lysates NM_001005377.
Western blot validation of overexpression lysate (LS005329) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PLAUR (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
PLAUR Human Over-expression Lysates NM_002659.
Western blot validation of overexpression lysate (LS005329) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PLAUR (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for PLAUR (Source: Uniprot.org, NCBI)
Gene Name
PLAUR
Full Name
Urokinase plasminogen activator surface receptor
Weight
36.978kDa
Alternative Names
CD87 antigen; CD87; Monocyte activation antigen Mo3; plasminogen activator, urokinase receptor; PLAUR; uPAR; U-PAR; UPARurokinase plasminogen activator surface receptor; u-plasminogen activator receptor form 2; URKRMO3 PLAUR CD87, U-PAR, UPAR, URKR plasminogen activator, urokinase receptor urokinase plasminogen activator surface receptor|monocyte activation Mo3|u-plasminogen activator receptor form 2|urokinase-type plasminogen activator (uPA) receptor
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on PLAUR, check out the PLAUR Infographic
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In this infographic, you will see the following information for PLAUR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For PLAUR (NM_002659) Human Over-expression Lysate (LS005329)
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