Product Info Summary
SKU: | LS009588 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of peroxiredoxin 6 (PRDX6) |
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Product info
Product Name
Peroxiredoxin 6 (PRDX6) (NM_004905) Human Over-expression Lysate
See all Peroxiredoxin 6 products
SKU/Catalog Number
LS009588
Size
100 µg
Description
Transient overexpression lysate of peroxiredoxin 6 (PRDX6)
Storage & Handling
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Cite This Product
Peroxiredoxin 6 (PRDX6) (NM_004905) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS009588)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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Peroxiredoxin 6 (PRDX6) Human Over-expression Lysates NM_004905.
Western blot validation of overexpression lysate (LS009588) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PRDX6 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for PRDX6 (Source: Uniprot.org, NCBI)
Gene Name
PRDX6
Full Name
Peroxiredoxin-6
Weight
Superfamily
peroxiredoxin family
Alternative Names
1-Cys; 24 kDa protein; Acidic calcium-independent phospholipase A2; aiPLA2; aiPLA21-Cys PRX; Antioxidant protein 2; AOP2; AOP2Non-selenium glutathione peroxidase; EC 1.11.1.15; EC 1.11.1.7; EC 3.1.1.-; KIAA0106Red blood cells page spot 12; Liver 2D page spot 40; MGC46173; NSGPx; NSGPx1-Cys peroxiredoxin; p29; Peroxiredoxin 6; peroxiredoxin-6; PRDX6; PRX PRDX6 1-Cys, AOP2, HEL-S-128m, LPCAT-5, NSGPx, PRX, aiPLA2, p29 peroxiredoxin 6 peroxiredoxin-6|1-Cys PRX|1-Cys peroxiredoxin|24 kDa protein|LPC acyltransferase 5|acidic calcium-independent phospholipase A2|antioxidant protein 2|epididymis secretory sperm binding protein Li 128m|glutathione-dependent peroxiredoxin|liver 2D page spot 40|lyso-PC acyltransferase 5|lysophosphatidylcholine acyltransferase 5|non-selenium glutathione peroxidase|red blood cells page spot 12
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on PRDX6, check out the PRDX6 Infographic
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In this infographic, you will see the following information for PRDX6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Peroxiredoxin 6 (PRDX6) (NM_004905) Human Over-expression Lysate (LS009588)
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