Anti-PRDX6/Peroxiredoxin 6 Rabbit Monoclonal Antibody

Product Name

Anti-PRDX6/Peroxiredoxin 6 Rabbit Monoclonal Antibody

View all Peroxiredoxin 6 Antibodies

SKU/Catalog Number

M01847

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-PRDX6/Peroxiredoxin 6 Rabbit Monoclonal Antibody catalog # M01847. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PRDX6/Peroxiredoxin 6 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01847)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AAGB-16

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human PRDX6

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01847 is reactive to PRDX6 in Human, Mouse, Rat

Applications

M01847 is guaranteed for IP, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

25 kDa

Calculated molecular weight

25035 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:1000
ICC/IF 1:50-1:200
IP 1:20

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of PRDX6 using anti-PRDX6 antibody (M01847).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDX6 antigen affinity purified polyclonal antibody (Catalog # M01847) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRDX6 at approximately 25 kDa. The expected band size for PRDX6 is at 25 kDa.

Gene/Protein Information For PRDX6 (Source: Uniprot.org, NCBI)

Alternative Names

1-Cys; 24 kDa protein; Acidic calcium-independent phospholipase A2; aiPLA2; aiPLA21-Cys PRX; Antioxidant protein 2; AOP2; AOP2Non-selenium glutathione peroxidase; EC 1.11.1.15; EC 1.11.1.7; EC 3.1.1.-; KIAA0106Red blood cells page spot 12; Liver 2D page spot 40; MGC46173; NSGPx; NSGPx1-Cys peroxiredoxin; p29; Peroxiredoxin 6; peroxiredoxin-6; PRDX6; PRX PRDX6 1-Cys, AOP2, HEL-S-128m, LPCAT-5, NSGPx, PRX, aiPLA2, p29 peroxiredoxin 6 peroxiredoxin-6|1-Cys PRX|1-Cys peroxiredoxin|24 kDa protein|LPC acyltransferase 5|acidic calcium-independent phospholipase A2|antioxidant protein 2|epididymis secretory sperm binding protein Li 128m|glutathione-dependent peroxiredoxin|liver 2D page spot 40|lyso-PC acyltransferase 5|lysophosphatidylcholine acyltransferase 5|non-selenium glutathione peroxidase|red blood cells page spot 12

For more info on PRDX6, check out the PRDX6 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PRDX6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the PRDX6 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis