PDGF-BB Platelet-Derived Growth Factor BB Rat Recombinant Protein

Product Name

PDGF-BB Platelet-Derived Growth Factor BB Rat Recombinant Protein

View all PDGFB recombinant proteins

SKU/Catalog Number

PROTQ05028

Size

2ug, 10ug, 1mg

Description

PDGF-BB Rat Recombinant produced in E.coli is a disulfide-linked homodimeric, non-glycosylated, polypeptide of two B chains containing 2x109 amino acids (218 amino acids in total) and having a molecular mass of 24.4 kDa. The PDGF-BB is purified by proprietary chromatographic techniques.

Storage & Handling

Lyophilized Platelet-derived Growth Factor BB although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF BB should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Cite This Product

PDGF-BB Platelet-Derived Growth Factor BB Rat Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTQ05028)

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

PDGF-BB was lyophilized from a 0.2µm filtered concentrated solution in 1xPBS, pH 7.0.

Purity

Greater than 98.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.

Predicted MW

27.283kDa

Reconstitution

It is recommended to reconstitute the lyophilized PDGF-BB in sterile 18M-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Biological Activity

The ED₅₀as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 2.0 ng/ml, corresponding to a specific activity of greater than 5.0 × 10⁵ IU/mg.

Reconstitution

It is recommended to reconstitute the lyophilized PDGF-BB in sterile 18M-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Validation Images & Assay Conditions

Gene/Protein Information For PDGFB (Source: Uniprot.org, NCBI)

Alternative Names

Platelet-derived growth factor subunit B; PDGF subunit B; PDGF-2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; Pdgfb; SIS; c-sis PDGFB IBGC5, PDGF-2, PDGF2, SIS, SSV, c-sis platelet derived growth factor subunit B platelet-derived growth factor subunit B|PDGF subunit B|PDGF, B chain|becaplermin|epididymis secretory sperm binding protein|platelet-derived growth factor 2|platelet-derived growth factor B chain|platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)|platelet-derived growth factor, beta polypeptide (oncogene SIS)|proto-oncogene c-Sis

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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