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Product Info Summary
| SKU: | PROTQ05028 |
|---|---|
| Size: | 2ug, 10ug, 1mg |
| Origin Species: | Rat |
| Source: | Escherichia coli |
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Product info
Product Name
PDGF-BB Platelet-Derived Growth Factor BB Rat Recombinant Protein
View all PDGFB recombinant proteins
SKU/Catalog Number
PROTQ05028
Size
2ug, 10ug, 1mg
Description
PDGF-BB Rat Recombinant produced in E.coli is a disulfide-linked homodimeric, non-glycosylated, polypeptide of two B chains containing 2x109 amino acids (218 amino acids in total) and having a molecular mass of 24.4 kDa. The PDGF-BB is purified by proprietary chromatographic techniques.
Storage & Handling
Lyophilized Platelet-derived Growth Factor BB although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF BB should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Cite This Product
PDGF-BB Platelet-Derived Growth Factor BB Rat Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTQ05028)
Form
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
PDGF-BB was lyophilized from a 0.2µm filtered concentrated solution in 1xPBS, pH 7.0.
Purity
Greater than 98.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.
Reconstitution
It is recommended to reconstitute the lyophilized PDGF-BB in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Amino Acid Sequence
SLGSLAAAEP AVIAECKTRT EVFQISRNLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RASQVQMRPV QVRKIEIVRK KPVFKKATVT LEDHLACKCE TVVTPRPVT
Biological Activity
The ED50as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 2.0 ng/ml, corresponding to a specific activity of greater than 5.0 × 105 IU/mg.
Assay dilution & Images
Reconstitution
It is recommended to reconstitute the lyophilized PDGF-BB in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Validation Images & Assay Conditions
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Recombinant protein fun image
Protein Target Info & Infographic
Gene/Protein Information For PDGFB (Source: Uniprot.org, NCBI)
Gene Name
PDGFB
Full Name
Platelet-derived growth factor subunit B
Weight
Superfamily
PDGF/VEGF growth factor family
Alternative Names
PDGFBB; PDGF-BB PDGFB IBGC5, PDGF-2, PDGF2, SIS, SSV, c-sis platelet derived growth factor subunit B platelet-derived growth factor subunit B|PDGF subunit B|PDGF, B chain|becaplermin|epididymis secretory sperm binding protein|platelet-derived growth factor 2|platelet-derived growth factor B chain|platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)|platelet-derived growth factor, beta polypeptide (oncogene SIS)|proto-oncogene c-Sis
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on PDGFB, check out the PDGFB Infographic
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In this infographic, you will see the following information for PDGFB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For PDGF-BB Platelet-Derived Growth Factor BB Rat Recombinant Protein (PROTQ05028)
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