PDGF-AA Platelet-Derived Growth Factor AA Rat Recombinant Protein

Product Name

PDGF-AA Platelet-Derived Growth Factor AA Rat Recombinant Protein

View all Pdgfa recombinant proteins

SKU/Catalog Number

PROTP28576

Size

2ug, 10ug, 1mg

Description

Platelet-derived Growth Factor AA Human Recombinant is a disulfide-linked homodimer Consists of two A chains containing 111 amino acids each and having a total molecular mass of 25.3KDa. PDGF-AA is purified by proprietary chromatographic techniques.

Storage & Handling

Lyophilized Platelet-derived Growth Factor AA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF-AA should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Cite This Product

PDGF-AA Platelet-Derived Growth Factor AA Rat Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP28576)

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

PDGF-AA was lyophilized from a 0.2µm filtered concentrated solution in PBS, pH7.0.

Purity

Greater than 97.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.

Predicted MW

24.043kDa

Reconstitution

It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-AA in Sterile 4mM HCl to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Biological Activity

The ED₅₀, as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 5.0 ng/ml, corresponding to a specific activity of greater than 2.0 × 10⁵ IU/mg.

Reconstitution

It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-AA in Sterile 4mM HCl to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Validation Images & Assay Conditions

Gene/Protein Information For PDGFA (Source: Uniprot.org, NCBI)

Alternative Names

Platelet-derived growth factor subunit A; PDGF subunit A; PDGF-1; Platelet-derived growth factor A chain; Platelet-derived growth factor alpha polypeptide; Pdgfa; Rpa1 PDGFA PDGF-A, PDGF1 platelet derived growth factor subunit A platelet-derived growth factor subunit A|PDGF A-chain|PDGF subunit A|platelet-derived growth factor A-chain|platelet-derived growth factor alpha chain|platelet-derived growth factor alpha polypeptide

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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