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- Table of Contents
2 Citations
Facts about Platelet-derived growth factor subunit A.
Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum.
Human | |
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Gene Name: | PDGFA |
Uniprot: | P04085 |
Entrez: | 5154 |
Belongs to: |
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PDGF/VEGF growth factor family |
PDGFAA; PDGF-AA
Mass (kDA):
24.043 kDA
Human | |
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Location: | 7p22.3 |
Sequence: | 7; NC_000007.14 (497245..520668, complement) |
Secreted. Released by platelets upon wounding.
You've probably wondered, whether you're a scientist, or a healthcare professional what the best uses for the PDGFA Marker could be. There are many options. Boster's products have been validated across multiple platforms using known positive and negatively sampled to ensure high specificity and high affinity. Boster also rewards scientists from around the world with product credits, giving them credit for their work.
PDGFA is a human growthhormone receptor. This protein can be found throughout a variety of tissues, including bone, skin, and kidneys. The PDGFA marker can be used to measure the levels of this protein in different biological systems. Researchers can use PDGFA to monitor growth in cancer cells and other types. The PDGFA gene also makes many other human growthhormones, such as the retinoic.
In addition to its potential for cancer cell research, PDGFR has also been shown to be an excellent marker for mesenchymal stem and progenitor cells. PDGFR+ cell lines are found in adult murine tissues. These cells can be distinguished by haematopoietic as well as endothelial populations. It has also been shown to be linked with the expression of CD51+ within BM cells.
PDGFA immunofluorescence methods differ from GFP immunofluorescence because of the marker's high affinity with intracellular proteins. Many fixation methods have been created in recent years to improve intracellular proteins accessibility. Common mitogens such as Ca++ and PMA may increase the production of cytokine proteins within cells. Protein transport inhibitors can be used to prevent the loss of proteins. However, they can make the detection process difficult if the proteins are released before the antibody is detected or degraded rapidly. Brefeldin, a Golgi inhibitor, can be used for this purpose to block the Golgi's secretion. This block traps secreted proteins and allows for immunofluorescence detection.
The PDGFs play a key role in tissue structure configuration and remodeling. PDGFA can be found in almost every mesenchymal cell type, including post-gastrulation mesoderm/neural crown stem cells. It is also present in progenitor cells for bone and teeth. The PDGFA marker, which is also found on pericytes, participates extracellular matrix remodeling as well as vascular bed maintenance.
The PDGFA mark is one of their key differences. Fluorescently conjugated antibodies are used to label cells for flow cytometric analysis in the PDGFA immunofluorescence technique. Fluorescently labeled antibodies interact with the light beam and generate a pulse. The area of the voltage pulse is directly related to the fluorescence level of the cells. The higher the fluorescence, the greater the channel count.
The PDGFA marker is a promising option for this immunofluorescence method. The PDGFA marker binds to cell surfaces with high affinity, which means that it is suitable for use in research and for clinical trials. PDGFA immunofluorescence presents a significant problem due to the lack of high-affinity prima antibodies. Biotin-conjugated antibodies can be used to address this problem.
The PDGFRA protein gene is highly variable. GISTs shows a specific example of PDGFRA mutation inactivation. A single sample of the PDGFRA gene reveals a deletion mutation at the DIMH gene. The heteroduplexes of wild-type PDGFRA protein and the deletion mutant are easily identified.
A genetically upregulated PDGF receptor version showed less inflammation and decreased fibrosis in a mouse model with heart failure. PDGFAB, a PDGFRa ligand which can activate endothelial and pericytes cells as well as fibroblasts, can also be used to directly activate fibroblasts. Studies have shown that PDGFRa overexpression under a-MHC promoter could lead to focal fibrosis or cardiac hypertrophy.
The PDGFRA protein is a type III receptor tyrosine kinase with homology to KIT, FLT3, CSF1-R, and PDGFR-b. It was first found in GISTs. The protein was then characterized in myeloproliferative disease. It was not used in myeloproliferative conditions until recently. The consensus sequence summarizes the regions of relatively active mutations.
PMID: 3422746 by Bonthron D.T., et al. Platelet-derived growth factor A chain: gene structure, chromosomal location, and basis for alternative mRNA splicing.
PMID: 2832727 by Rorsman F., et al. Structural characterization of the human platelet-derived growth factor A-chain cDNA and gene: alternative exon usage predicts two different precursor proteins.
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