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Product Info Summary
| SKU: | EKC2489 |
|---|---|
| Size: | 1 kit, containing two 96-well plates and all necessary reagents |
| Reactive Species: | Human, Mouse, Rat |
| Application: | ELISA |
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Product info
Product Name
p70 S6 Kinase (Phospho-Ser424) Colorimetric Cell-Based ELISA Kit
See all p70 S6 Kinase/S6K products
SKU/Catalog Number
EKC2489
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The p70 S6 Kinase (Phospho-Ser424) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor p70 S6 Kinase (Phospho-Ser424) protein expression profile in cells. The kit can be used for measuring the relative amounts of p70 S6 Kinase (Phospho-Ser424) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on p70 S6 Kinase (Phospho-Ser424).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
p70 S6 Kinase (Phospho-Ser424) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2489)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC2489 is reactive to RPS6KB1 in Human, Mouse, Rat samples
Application Guarantee
EKC2489 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 2 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-p70 S6 Kinase Phospho-Ser424 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Red |
| 100x Anti-p70 S6 Kinase Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 12ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 12ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 12ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from A549 cells treated with PMA 125ng/ML 30'
Protein Target Info & Infographic
Gene/Protein Information For RPS6KB1 (Source: Uniprot.Org, NCBI)
Gene Name
RPS6KB1
Full Name
Ribosomal protein S6 kinase beta-1
Weight
59140 MW
Superfamily
protein kinase superfamily
Alternative Names
Ribosomal protein S6 kinase beta-1;S6K-beta-1;S6K1;2.7.11.1;70 kDa ribosomal protein S6 kinase 1;P70S6K1;p70-S6K 1;Ribosomal protein S6 kinase I;Serine/threonine-protein kinase 14A;p70 ribosomal S6 kinase alpha;p70 S6 kinase alpha;p70 S6K-alpha;p70 S6KA;RPS6KB1;STK14A; RPS6KB1 PS6K, S6K, S6K-beta-1, S6K1, STK14A, p70 S6KA, p70(S6K)-alpha, p70-S6K, p70-alpha ribosomal protein S6 kinase B1 ribosomal protein S6 kinase beta-1|ribosomal protein S6 kinase I|ribosomal protein S6 kinase, 70kDa, polypeptide 1|serine/threonine kinase 14 alpha|serine/threonine-protein kinase 14A
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on RPS6KB1, check out the RPS6KB1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for RPS6KB1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
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