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Product Info Summary
| SKU: | P01475 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-Phospho-S6K1 (T421 + S424) RPS6KB1 Rabbit Monoclonal Antibody
View all p70 S6 Kinase/S6K Antibodies
SKU/Catalog Number
P01475
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-S6K1 (T421 + S424) RPS6KB1 Rabbit Monoclonal Antibody catalog # P01475. Tested in WB, IP applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-S6K1 (T421 + S424) RPS6KB1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01475)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BHD-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-S6K1 (T421 + S424)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P01475 is reactive to RPS6KB1 in Human, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
59140 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P01475 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IP 1:30
Positive Control
WB: HEK293 cell
Validation Images & Assay Conditions
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Western blot analysis of SK61 phosphorylation expression in HEK293 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For RPS6KB1 (Source: Uniprot.org, NCBI)
Gene Name
RPS6KB1
Full Name
Ribosomal protein S6 kinase beta-1
Weight
59140 MW
Superfamily
protein kinase superfamily
Alternative Names
Ribosomal protein S6 kinase beta-1;S6K-beta-1;S6K1;2.7.11.1;70 kDa ribosomal protein S6 kinase 1;P70S6K1;p70-S6K 1;Ribosomal protein S6 kinase I;Serine/threonine-protein kinase 14A;p70 ribosomal S6 kinase alpha;p70 S6 kinase alpha;p70 S6K-alpha;p70 S6KA;RPS6KB1;STK14A; RPS6KB1 PS6K, S6K, S6K-beta-1, S6K1, STK14A, p70 S6KA, p70(S6K)-alpha, p70-S6K, p70-alpha ribosomal protein S6 kinase B1 ribosomal protein S6 kinase beta-1|ribosomal protein S6 kinase I|ribosomal protein S6 kinase, 70kDa, polypeptide 1|serine/threonine kinase 14 alpha|serine/threonine-protein kinase 14A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPS6KB1, check out the RPS6KB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPS6KB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-S6K1 (T421 + S424) RPS6KB1 Rabbit Monoclonal Antibody (P01475)
Hello CJ!
P01475 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xu R,Zhang Y,Li A,Ma Y,Cai W,Song L,Xie Y,Zhou S,Cao W,Tang X.LY‑294002 enhances the chemosensitivity of liver cancer to oxaliplatin by blocking the PI3K/AKT/HIF‑1α pathway.Mol Med Rep.2021 Jul;24(1):508.doi:10.3892/mmr.2021.12147.Epub 2021 May 13.PMID:33982772.
Species: Human
P01475 usage in article: APP:WB, SAMPLE:HEPG-2 CELL, DILUTION:NA
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Customer Q&As
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1 Customer Q&As for Anti-Phospho-S6K1 (T421 + S424) RPS6KB1 Rabbit Monoclonal Antibody
Question
We are currently using anti-Phospho-S6K1 (T421 + S424) Rabbit Monoclonal antibody P01475 for rat tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on zebrafish tissues as well?
H. Krishna
Verified customer
Asked: 2014-09-26
Answer
The anti-Phospho-S6K1 (T421 + S424) Rabbit Monoclonal antibody (P01475) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-09-26
