Product Info Summary
SKU: | M01475-1 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IP, IHC, WB |
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Product info
Product Name
Anti-S6K1 RPS6KB1 Rabbit Monoclonal Antibody
View all p70 S6 Kinase/S6K Antibodies
SKU/Catalog Number
M01475-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-S6K1 RPS6KB1 Rabbit Monoclonal Antibody catalog # M01475-1. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-S6K1 RPS6KB1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01475-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CDB-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human S6K1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01475-1 is reactive to RPS6KB1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
51 kDa
Calculated molecular weight
59140 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01475-1 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
Positive Control
WB: 293T cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of S6K1 expression in 293T cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For RPS6KB1 (Source: Uniprot.org, NCBI)
Gene Name
RPS6KB1
Full Name
Ribosomal protein S6 kinase beta-1
Weight
59140 MW
Superfamily
protein kinase superfamily
Alternative Names
Ribosomal protein S6 kinase beta-1;S6K-beta-1;S6K1;2.7.11.1;70 kDa ribosomal protein S6 kinase 1;P70S6K1;p70-S6K 1;Ribosomal protein S6 kinase I;Serine/threonine-protein kinase 14A;p70 ribosomal S6 kinase alpha;p70 S6 kinase alpha;p70 S6K-alpha;p70 S6KA;RPS6KB1;STK14A; RPS6KB1 PS6K, S6K, S6K-beta-1, S6K1, STK14A, p70 S6KA, p70(S6K)-alpha, p70-S6K, p70-alpha ribosomal protein S6 kinase B1 ribosomal protein S6 kinase beta-1|ribosomal protein S6 kinase I|ribosomal protein S6 kinase, 70kDa, polypeptide 1|serine/threonine kinase 14 alpha|serine/threonine-protein kinase 14A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPS6KB1, check out the RPS6KB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPS6KB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-S6K1 RPS6KB1 Rabbit Monoclonal Antibody (M01475-1)
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No publications found for M01475-1
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Customer Q&As
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6 Customer Q&As for Anti-S6K1 RPS6KB1 Rabbit Monoclonal Antibody
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-S6K1 Rabbit Monoclonal antibody M01475-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-07
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-07
Question
Does anti-S6K1 Rabbit Monoclonal antibody M01475-1 work on monkey IP with cerebellar vermis?
K. Zhao
Verified customer
Asked: 2018-09-24
Answer
Our lab technicians have not tested anti-S6K1 Rabbit Monoclonal antibody M01475-1 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-S6K1 Rabbit Monoclonal antibody M01475-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey cerebellar vermis in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-24
Question
Would anti-S6K1 Rabbit Monoclonal antibody M01475-1 work for WB with cervix carcinoma?
Z. Lewis
Verified customer
Asked: 2018-04-13
Answer
According to the expression profile of cervix carcinoma, RPS6KB1 is highly expressed in cervix carcinoma. So, it is likely that anti-S6K1 Rabbit Monoclonal antibody M01475-1 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2018-04-13
Question
I was wanting to use your anti-S6K1 Rabbit Monoclonal antibody for WB for human cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2017-07-26
Answer
It shows on the product datasheet, M01475-1 anti-S6K1 Rabbit Monoclonal antibody has been validated for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-07-26
Question
Is a blocking peptide available for product anti-S6K1 Rabbit Monoclonal antibody (M01475-1)?
L. Anderson
Verified customer
Asked: 2017-07-17
Answer
We do provide the blocking peptide for product anti-S6K1 Rabbit Monoclonal antibody (M01475-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-07-17
Question
I am looking for to test anti-S6K1 Rabbit Monoclonal antibody M01475-1 on human cervix carcinoma for research purposes, then I may be interested in using anti-S6K1 Rabbit Monoclonal antibody M01475-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Li
Verified customer
Asked: 2016-05-24
Answer
The products we sell, including anti-S6K1 Rabbit Monoclonal antibody M01475-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-05-24