ELISA Technical Blogs

5 Pitfalls to Avoid for ELISA!

November 24, 2017

Keywords: troubleshooting tips, technical, optimization

ELISA is a convenient and simple method to quantitatively or qualitatively detect peptides, proteins, antibodies, and hormones in samples, rendering it as one of the most widely used immunoassays. Despite the many advantages of conducting ELISA, there are some mistakes that could quickly turn your ELISA experiment sour.

Read about the 5 common pitfalls to avoid when performing an ELISA so that you can prevent this situation from happening!

Which ELISA is for you?

July 28, 2017

Keywords: ELISA protocol, comparison table, technical tips, ELISA assay format, advantages of sandwich ELISA, indirect, direct, competitive immunoassay

Are you familiar with the multiple methods you could use to perform an ELISA? Among the standard assay formats, it is important to differentiate between the particular strategies that exist specifically for the detection step. An antigen could be captured to the plate by direct adsorption to the surface or through a pre-coated "capture" antibody, as in a sandwich ELISA. However, it is the detection step (direct or indirect detection) that largely determines the sensitivity of an ELISA. Read on to discover which ELISA is for you...

How to Use Checkerboard Titration to Optimize your ELISA Immunoassays

June 16, 2017

Keywords: ELISA assay optimization tips, checkboard titration principle explanation, ELISA protocol optimize, how to titrate

Optimizing your ELISA immunoassay can be a long and tedious process. Fortunately, using checkboard titration could help you optimize your ELISA assay more quickly, efficiently, and accurately. Here is a how-to-guide for utilizing this method in your next ELISA protocol.

ELISA Troubleshooting Saturated Signals

Apr 28, 2017

Keywords: ELISA immunoassay, technical tips, troubleshooting, ELISA excess signal, ELISA kit result optimization

Every ELISA kit user comes across the need to troubleshoot their assay at some point. Saturated signals can result from many different possible causes, and this could make troubleshooting complicated. Dr. Booster has come up with a table of recommended solutions for troubleshooting your ELISA assay. If this table does not help your specific experiment, please feel free to contact Boster's technical support at any time. Get better results with Boster!