Multiplex ELISA Kit For Human Micronutrient Array (7-plex)

ELISA kit for Multiplex detection

The Multiplex Human Micronutrient Array (7-plex) is a fully quantitative chemiluminescent assay allowing concurrent measurement of seven biomarkers: AGP, CRP, Ferritin, HRP2, RBP4, sTfR, Thyroglobulin. Attention: This product is from Quansys Bio and requires a special plate reader. Please make sure you have the proper equipment before ordering. For details, please contact [email protected].

Product Info Summary

Species: Human
Sample Type: Serum and Heparin Plasma
Volume Required per Well: Min 5 µl
Assay Length: 3.5 hours
Specificity: All analytes < 1% intra-panel cross-reactivity
Assay Type: Planar Based Multiplexed ELISA
Detection Method: Chemiluminescent
Multiplex Format: 96-well solid plate
Catalog: MEK1024
Manufacturer Reference Number: 355149HU
Analytes:AGP, CRP, Ferritin, HRP2, RBP4, sTfR, Thyroglobulin

Product Overview

The Multiplex Human Micronutrient Array (7-plex) is a fully quantitative chemiluminescent assay allowing concurrent measurement of biomarkers used in nutritional assessment in serum or heparin plasma samples. Analytes CRP, Ferritin, HRP2, sTfR, and Thyroglobulin (Tg) are sandwich ELISAs while AGP and RBP4 are competitive ELISAs. This product is alternatively known in previous publications as Multiplex Micronutrient Assessment Tool (MMAT).

A Human Micronutrient Assay kit contains a 96-well plate, with each well featuring the 7-plex along with reagents necessary to perform the test. Our high quality reagents help ensure the accuracy of your results.

Using just 5 µl of sample per well, up to 80 wells can be tested for all seven markers in the panel in 3.5 hours. Multiplex Arrays provide scientists with an easy-to-use and cost effective means of generating a biomarker profile for each sample.

Assay Ranges

AnalytesAssay TypesCalibrator RangeUpper Limit of Quantification (ULOQ)Lower Limit of Quantification (LLOQ)Limit of Detection
AGPCompetitive0.36-0.0005 (g/L)0.25 (g/L)0.0019 (g/L)0.00096 (g/L)
CRPSandwich1-0.0014 (mg/L)0.7 (mg/L)0.0018 (mg/L)0.00089 (mg/L)
FerritinSandwich23-0.032 (µg/L)16.17 (µg/L)0.041 (µg/L)0.021 (µg/L)
HRP2Sandwich1.9-0.0026 (µg/L)1.33 (µg/L)0.0034 (µg/L)0.0017 (µg/L)
RBP4Competitive1.01-0.0014 (µmol/L)0.71 (µmol/L)0.0054 (µmol/L)0.0027 (µmol/L)
sTFRSandwich4-0.0055 (mg/L)2.8 (mg/L)0.0071 (mg/L)0.0036 (mg/L)
ThyroglobulinSandwich15.1-0.021 (µg/L)10.57 (µg/L)0.027 (µg/L)0.013 (µg/L)

Kit Components

Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization.

Reagents Include:

Calibrator: Lyophilized, recombinant antigens in a buffered protein base
Calibrator Additive: Purified ferritin antigen in a buffered protein base
Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives
Competitor: Lyophilized, biotinylated competitive antigen
Substrate A: Liquid, 3.5 mL/vial
Substrate B: Liquid, 3.5 mL/vial
Sample Diluent (2X): Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives
Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
Plate Seals (3): Adhesive strips

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Plate Reader Option

Total: $1700

SKU:MEK1024

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Lead time for this item is typically 10-14 days

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MEK1024
The multiplex ELISA kits require special plate readers. For more info see special plate reader. The $800 demo option is for new customer only, see more info at demo options.
$1,700.00

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