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Product Info Summary
| Species: | Human |
|---|---|
| Sample Type: | Serum and Heparin Plasma |
| Volume Required per Well: | Min 5 µl |
| Assay Length: | 3.5 hours |
| Specificity: | All analytes < 1% intra-panel cross-reactivity |
| Assay Type: | Planar Based Multiplexed ELISA |
| Detection Method: | Chemiluminescent |
| Multiplex Format: | 96-well solid plate |
| Catalog: | MEK1024 |
| Manufacturer Reference Number: | 355149HU |
| Analytes: | AGP, CRP, Ferritin, HRP2, RBP4, sTfR, Thyroglobulin |
Product info
Product Overview
The Multiplex Human Micronutrient Array (7-plex) is a fully quantitative chemiluminescent assay allowing concurrent measurement of biomarkers used in nutritional assessment in serum or heparin plasma samples. Analytes CRP, Ferritin, HRP2, sTfR, and Thyroglobulin (Tg) are sandwich ELISAs while AGP and RBP4 are competitive ELISAs. This product is alternatively known in previous publications as Multiplex Micronutrient Assessment Tool (MMAT).
A Human Micronutrient Assay kit contains a 96-well plate, with each well featuring the 7-plex along with reagents necessary to perform the test. Our high quality reagents help ensure the accuracy of your results.
Using just 5 µl of sample per well, up to 80 wells can be tested for all seven markers in the panel in 3.5 hours. Multiplex Arrays provide scientists with an easy-to-use and cost effective means of generating a biomarker profile for each sample.
Assay Ranges
| Analytes | Assay Types | Calibrator Range | Upper Limit of Quantification (ULOQ) | Lower Limit of Quantification (LLOQ) | Limit of Detection |
|---|---|---|---|---|---|
| AGP | Competitive | 0.36-0.0005 (g/L) | 0.25 (g/L) | 0.0019 (g/L) | 0.00096 (g/L) |
| CRP | Sandwich | 1-0.0014 (mg/L) | 0.7 (mg/L) | 0.0018 (mg/L) | 0.00089 (mg/L) |
| Ferritin | Sandwich | 23-0.032 (µg/L) | 16.17 (µg/L) | 0.041 (µg/L) | 0.021 (µg/L) |
| HRP2 | Sandwich | 1.9-0.0026 (µg/L) | 1.33 (µg/L) | 0.0034 (µg/L) | 0.0017 (µg/L) |
| RBP4 | Competitive | 1.01-0.0014 (µmol/L) | 0.71 (µmol/L) | 0.0054 (µmol/L) | 0.0027 (µmol/L) |
| sTFR | Sandwich | 4-0.0055 (mg/L) | 2.8 (mg/L) | 0.0071 (mg/L) | 0.0036 (mg/L) |
| Thyroglobulin | Sandwich | 15.1-0.021 (µg/L) | 10.57 (µg/L) | 0.027 (µg/L) | 0.013 (µg/L) |
Kit Components
Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization.
Reagents Include:
Calibrator: Lyophilized, recombinant antigens in a buffered protein base
Calibrator Additive: Purified ferritin antigen in a buffered protein base
Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives
Competitor: Lyophilized, biotinylated competitive antigen
Substrate A: Liquid, 3.5 mL/vial
Substrate B: Liquid, 3.5 mL/vial
Sample Diluent (2X): Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives
Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
Plate Seals (3): Adhesive strips
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