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Product Info Summary
| Species: | Human |
|---|---|
| Sample Type: | Serum, EDTA/Heparin Plasma |
| Volume Required per Well: | Min 25 µl |
| Assay Length: | 2.25 hours |
| Specificity: | All analytes < 1% intra-panel cross-reactivity |
| Assay Type: | Planar Based Multiplexed ELISA |
| Detection Method: | Chemiluminescent |
| Multiplex Format: | 96-well solid plate |
| Catalog: | MEK1001 |
| Manufacturer Reference Number: | 110333HU |
| Analytes: | IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-15, IL-17A, IL-23, TNFα, TNFβ |
Product info
Product Overview
The Multiplex Human Cytokine – Stripwells (16-plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 16 biomarkers or analytes. Using only 25 µl of sample per well, up to 80 samples can be assayed for all 16 markers in the panel within 2.25 hours.
The Multiplex Human Cytokine HS Screen (15-Plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing 16 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.
Assay Ranges
| Analyte | Assay Type | Calibrator Range | Upper Limit of Quantification (ULOQ) | Lower Limit of Quantification (LLOQ) | Limit of Detection |
|---|---|---|---|---|---|
| IFNγ | Sandwich | 8000 -10.97 (pg/mL) | 5600 (pg/mL) | 14.3 (pg/mL) | 4.95 (pg/mL) |
| IL-1α | Sandwich | 4000 -5.49 (pg/mL) | 2800 (pg/mL) | 7.1 (pg/mL) | 5.43 (pg/mL) |
| IL-1β | Sandwich | 12226 -16.77 (pg/mL) | 8558 (pg/mL) | 21.8 (pg/mL) | 12.40 (pg/mL) |
| IL-2 | Sandwich | 5213 – 7.15 (pg/mL) | 3649 (pg/mL) | 9.3 (pg/mL) | 4.12 (pg/mL) |
| IL-4 | Sandwich | 1676 – 2.30 (pg/mL) | 1173 (pg/mL) | 3.0 (pg/mL) | 1.79 (pg/mL) |
| IL-5 | Sandwich | 3000 – 4.12 (pg/mL) | 2100 (pg/mL) | 5.3 (pg/mL) | 2.90 (pg/mL) |
| IL-6 | Sandwich | 3000 – 4.12 (pg/mL) | 2100 (pg/mL) | 5.3 (pg/mL) | 3.47 (pg/mL) |
| IL-8 | Sandwich | 2000 – 2.74 (pg/mL) | 1400 (pg/mL) | 3.6 (pg/mL) | 1.37 (pg/mL) |
| IL-10 | Sandwich | 8039 – 11.03 (pg/mL) | 5627 (pg/mL) | 14.3 (pg/mL) | 10.99 (pg/mL) |
| IL-12p70 | Sandwich | 3000 – 4.12 (pg/mL) | 2100 (pg/mL) | 5.3 (pg/mL) | 3.20 (pg/mL) |
| IL-13 | Sandwich | 3000 – 4.12 (pg/mL) | 2100 (pg/mL) | 5.3 (pg/mL) | 4.12 (pg/mL) |
| IL-15 | Sandwich | 10733 – 14.72 (pg/mL) | 7513 (pg/mL) | 19.1 (pg/mL) | 6.87 (pg/mL) |
| IL-17A | Sandwich | 16772 – 23.01 (pg/mL) | 11740 (pg/mL) | 29.9 (pg/mL) | 22.57 (pg/mL) |
| IL-23 | Sandwich | 41700 – 57.20 (pg/mL) | 21000 (pg/mL) | 120 (pg/mL) | 48.65 (pg/mL) |
| TNFα | Sandwich | 6500 – 8.92 (pg/mL) | 4522 (pg/mL) | 11.5 (pg/mL) | 7.74 (pg/mL) |
| TNFβ | Sandwich | 4000 – 5.49 (pg/mL) | 2800 (pg/mL) | 7.1 (pg/mL) | 5.29 (pg/mL) |
Kit Components
Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization.
Reagents Include:
Calibrator: Lyophilized, recombinant antigens in a buffered protein base
Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives
Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide
Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer
Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives
Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
Plate Seals (3): Adhesive strips
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6 Customer Q&As for Multiplex ELISA Kit For Human Cytokine - Stripwells (16-plex)
Question
Can bioteck plate reader be used for Multiplex ELISA Kit For Human Cytokine - Stripwells (16-Plex) (MEK1001)?
Verified customer
Asked: 2022-03-24
Answer
The minimum qualifications that the imager must meet to be able to image our plates are the following: 1. The imager has a light‐tight imaging chamber, a non‐illumination or chemi-luminescent mode, and can export grayscale 16‐bit TIFF files. 2. Camera‐based imagers must also have at least 1 MP resolution (4+ MP, flat‐field correction, and a cooled sensor is preferred). 3. In addition, the device must also be able to export grayscale 16-bit TIFF files. We require that customers purchase an additional substrate vial for third party imagers. If the customer will not be using a Q-View imager in the future, they need to purchase an additional substrate vial.
Boster Scientific Support
Answered: 2022-03-24
Question
Is it possible to use cell culture supernatants as the sample type for MEK1001?
Verified customer
Asked: 2020-12-18
Answer
Yes, you can use cell culture supernatants on the Multiplex ELISA Kit For Human Cytokine - Stripwells (16-Plex) (MEK1001).
Boster Scientific Support
Answered: 2020-12-21
Question
What kind of plate reader does MEK1001 require?
Verified customer
Asked: 2020-12-18
Answer
The Multiplex ELISA Kit For Human Cytokine - Stripwells (16-Plex) (MEK1001) is built to be used with Q-View imagers.
Boster Scientific Support
Answered: 2020-12-21
Question
Can those buffers in MEK1001 use separation and do independent experiment different times?
Verified customer
Asked: 2020-06-20
Answer
Yes, there are three separate vials that the customer would reconstitute one at a time as they need to use them. There are enough reagents to run the Multiplex ELISA Kit For Human Cytokine - Stripwells (16-Plex) (MEK1001) kit three different times.
Boster Scientific Support
Answered: 2020-06-23
Question
What plate reader is needed for the MEK1001 kit? Is this compatible with BioTek Synergy HT?
Verified customer
Asked: 2019-12-18
Answer
We do not support third party imagers and since there are so many models to keep track of, we are not certain which ones are compatible with our kits. The following are the requirements for a third party imager to be compatible with our plates: -The imager should have a light‐tight imaging chamber, a non‐illumination or chemi-luminescent mode, and can export grayscale 16‐bit TIFF files. -Camera‐based imagers must also have at least 1 MP resolution (4+ MP, flat‐field correction, and a cooled sensor is preferred).
Boster Scientific Support
Answered: 2019-12-23
Question
Does the MEK1001 kit come with any analysis software or is it not needed?
Verified customer
Asked: 2019-12-18
Answer
Unforunately not. The software must be purchased for $1,980 for long-term use (license does not expire). A free 60 day trial can be downloaded once you purchase the product.
Boster Scientific Support
Answered: 2019-12-23
