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Product Info Summary
| Species | Human |
| Sample Type | Human Serum, EDTA/Heparin Plasma |
| Volume Required Per Well | Min 25 µl |
| Assay Length | 4.83 hours |
| Specificity | All analytes < 1% intra-panel cross-reactivity |
| Assay Type | Sandwich |
| Detection Method | Chemiluminescent |
| Multiplex Format | 96-well solid plate |
| Catalog No | MEK2007 |
| Manufacturer Reference Number | 119733HU |
| Analytes | GMCSF, IFNα, IFNγ, IL-1β, IL-1RA, IL-2, IL-2Rα, IL-6, IL-6R, IL-8, IL-10, IL-12p70, IL-13, MCP-1, MIP-1α, TNFα |
Product info
Product Overview
The Multiplex ELISA Kit For Human Cytokine Release Syndrome (16-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 16 biomarkers or analytes. Using only 25 µl of sample per well, up to 80 samples can be assayed for all 16 markers in the panel within 4.83 hours.
The Multiplex ELISA Kit For Human Cytokine Release Syndrome (16-Plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing 16 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.
Assay Ranges
| Analytes | Calibrator Range | Units | LLD |
|---|---|---|---|
| GMCSF | 6,000-10.70 | pg/mL | 5.35 |
| IFNα | 400-0.72 | pg/mL | 0.36 |
| IFNγ | 99-0.18 | pg/mL | 0.09 |
| IL-1β | 4,075-7.27 | pg/mL | 3.64 |
| IL-1RA | 7,000-12.48 | pg/mL | 6.24 |
| IL-2 | 64-0.11 | pg/mL | 0.06 |
| IL-2Rα | 16,000-28.5 | pg/mL | 14.25 |
| IL-6 | 333-0.59 | pg/mL | 0.3 |
| IL-6R | 1,250-2.23 | pg/mL | 1.11 |
| IL-8 | 2,000-3.6 | pg/mL | 1.8 |
| IL-10 | 2,680-4.78 | pg/mL | 2.39 |
| IL-12p70 | 3,000-5.35 | pg/mL | 2.68 |
| IL-13 | 333-0.59 | pg/mL | 0.3 |
| MCP-1 | 2,000-3.6 | pg/mL | 1.8 |
| MIP-1α | 2,000-3.57 | pg/mL | 1.78 |
| TNFα | 661-1.18 | pg/mL | 0.59 |
Kit Components
Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing.
Reagents include:
Wash Buffer Concentrate (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
Sample Diluent: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives
Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered solution with preservatives
Calibrator: Lyophilized, recombinant antigens in a buffered protein base
Streptavidin-HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide
Substrate B+: Liquid, 3 mL/vial of stabilized signal enhancer
Plate Seals (3): Adhesive strips
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