Mouse TNF beta ELISA Kit PicoKine®

LTA ELISA kit for Mouse

Mouse TNF Beta ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Lta in cell culture supernatants, serum and plasma (heparin, EDTA or citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Info Summary

SKU: EK0585
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA or citrate).

Product Name

Mouse TNF beta ELISA Kit PicoKine®

View all LTA ELISA kits

SKU/Catalog Number

EK0585

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse TNF Beta ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Lta in cell culture supernatants, serum and plasma (heparin, EDTA or citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse TNF beta ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0585)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: E.coli; Immunogen sequence: L34-L202

Sensitivity

<10 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

See datasheet of EK0585 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0585 is reactive to Lta in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA or citrate).

Application Guarantee

EK0585 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of LTA

The encoded protein, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4, myocardial infarction, non-Hodgkin's lymphoma, and psoriatic arthritis. Alternatively spliced transcript variants have been observed for this gene.

Kit Components

Catalog Number Description Quantity
EK0585-CAP Anti-Mouse Lta Pre-coated 96-well strip microplate 1
EK0585-ST Mouse Lta Standard 2 vials, 10 ng/tube
EK0585-DA Mouse Lta Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.0350.0810.1400.2830.5251.0361.4682.011

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse TNF beta ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)913791647854601810
Standard deviation1.83/td>22.1098.332.4829.30110.05
CV (%)2.0%5.8%%6.0%2.9%6.4%6.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of LTA in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 1911069387942.132.3%
Sample 237932230135233918.355.4%
Sample 31647189512241470155946.153.0%
*number of samples for each test n=16.

Gene/Protein Information For Lta (Source: Uniprot.Org, NCBI)

Gene Name

Lta

Full Name

Lymphotoxin-alpha

Weight

22 kDa

Superfamily

tumor necrosis factor family

Alternative Names

LT; LTA; LT-alpha; lymphotoxin alpha (TNF superfamily, member 1); Lymphotoxin alpha; Lymphotoxinalpha; Lymphotoxin-alpha; tnfb; TNF-beta; TNFBlymphotoxin-alpha; TNFSF1; TNFSF1B; TNFSF1TNF-beta; tumor necrosis factor beta; Tumor necrosis factor ligand superfamily member 1 LTA LT, TNFB, TNFSF1, TNLG1E lymphotoxin alpha lymphotoxin-alpha|LT-alpha|TNF superfamily, member 1|TNF-beta|truncated lymphotoxin alpha|tumor necrosis factor beta|tumor necrosis factor ligand 1E|tumor necrosis factor ligand superfamily member 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Lta, check out the Lta Infographic

Lta infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Lta: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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