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Product Info Summary
| SKU: | EK2184 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse, Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Mouse/Rat DCC ELISA Kit PicoKine®
SKU/Catalog Number
EK2184
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse/Rat DCC ELISA Kit PicoKine® (96 Tests). Quantitate Mouse/Rat Dcc in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse/Rat DCC ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2184)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: F32-N1097
Sensitivity
<10 pg/ml
Assay Range
23.4 pg/ml - 1,500 pg/ml
Standard Dilution Instructions
See datasheet of EK2184 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2184 is reactive to Dcc in Mouse, Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2184 is guaranteed for ELISA in Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of DCC
Netrin receptor DCC, also known as DCC, or colorectal cancer suppressor is a protein which in humans is encoded by the DCC gene. Predicted to enable identical protein binding activity; netrin receptor activity; and transcription coactivator activity. Involved in postsynaptic modulation of chemical synaptic transmission. Acts upstream of or within generation of neurons. Located in axon. Is integral component of postsynaptic density membrane. Is active in Schaffer collateral - CA1 synapse. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; lung; and retina. Human ortholog(s) of this gene implicated in colorectal cancer; congenital mirror movement disorder; esophageal cancer; and esophagus squamous cell carcinoma. Orthologous to human DCC (DCC netrin 1 receptor).
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2184-CAP | Anti-Mouse, Rat Dcc Pre-coated 96-well strip microplate | 1 |
| EK2184-ST | Mouse, Rat Dcc Standard | 2 vials, 10 ng/tube |
| EK2184-DA | Mouse, Rat Dcc Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 23.4 | 46.8 | 93.7 | 187.5 | 375 | 750 | 1,500 |
| O.D. | 0.101 | 0.182 | 0.332 | 0.452 | 0.956 | 1.135 | 1.525 | 2.112 |
Data Example Images
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Mouse/Rat DCC PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse/Rat DCC ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 13 | 98 | 265 | 19 | 103 | 282 |
| Standard deviation | 0.81 | 6.17 | 15.64 | 0.91 | 5.25 | 15.51 |
| CV(%) | 6.2% | 6.3% | 5.9% | 4.8% | 5.1% | 5.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of DCC in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 13 | 15 | 18 | 22 | 17 | 1.17 | 6.9% |
| Sample 2 | 98 | 102 | 113 | 97 | 103 | 6.66 | 6.5% |
| Sample 3 | 265 | 278 | 296 | 282 | 280 | 18.5 | 6.6% |
Protein Target Info & Infographic
Gene/Protein Information For Dcc (Source: Uniprot.Org, NCBI)
Gene Name
Dcc
Full Name
Netrin receptor DCC
Weight
158.457kDa
Superfamily
immunoglobulin superfamily
Alternative Names
Colorectal cancer suppressor; CRC18; CRCR1; DCC; deleted in colorectal cancer protein; deleted in colorectal carcinoma; IGDCC1colorectal tumor suppressor; Immunoglobulin superfamily DCC subclass member 1; immunoglobulin superfamily, DCC subclass, member 1; netrin receptor DCC; Tumor suppressor protein DCC DCC CRC18, CRCR1, HGPPS2, IGDCC1, MRMV1, NTN1R1 DCC netrin 1 receptor netrin receptor DCC|colorectal cancer suppressor|deleted in colorectal carcinoma|immunoglobulin superfamily DCC subclass member 1|putative colorectal tumor suppressor|tumor suppressor protein DCC
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Dcc, check out the Dcc Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Dcc: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse/Rat DCC ELISA Kit PicoKine® (EK2184)
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