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Product Info Summary
| SKU: | A02490-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-PDE5A Antibody Picoband®
SKU/Catalog Number
A02490-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PDE5A Antibody Picoband® catalog # A02490-2. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PDE5A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02490-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human PDE5A, different from the related mouse sequence by eight amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A02490-2 is reactive to PDE5A in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
100 kDa
Calculated molecular weight
99985 MW
Background of PDE5A
cGMP-specific phosphodiesterase type 5 is an enzyme from the phosphodiesterase class. It is found in various tissues, most prominently the corpus cavernosum and the retina. It has also been recently discovered to play a vital role in the cardiovascular system. Furthermore, PDE5A also plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This PDE5A gene is mapped to 4q26.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02490-2 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: rat lung tissue, PANC-1 whole cell
IHC: human thyroid cancer tissue
Validation Images & Assay Conditions
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Figure 2. IHC analysis of PDE5A using anti-PDE5A antibody (A02490-2).
PDE5A was detected in paraffin-embedded section of human thyroid cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PDE5A Antibody (A02490-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 1. Western blot analysis of PDE5A using anti-PDE5A antibody (A02490-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
lane 1: rat lung tissue lysates,
lane 2: PANC-1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDE5A antigen affinity purified polyclonal antibody (Catalog # A02490-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDE5A at approximately 100KD. The expected band size for PDE5A is at 100KD.
Protein Target Info & Infographic
Gene/Protein Information For PDE5A (Source: Uniprot.org, NCBI)
Gene Name
PDE5A
Full Name
cGMP-specific 3',5'-cyclic phosphodiesterase
Weight
99985 MW
Superfamily
cyclic nucleotide phosphodiesterase family
Alternative Names
cGMP-specific 3',5'-cyclic phosphodiesterase;3.1.4.35 ;cGMP-binding cGMP-specific phosphodiesterase;CGB-PDE;PDE5A;PDE5; PDE5A CGB-PDE, CN5A, PDE5 phosphodiesterase 5A cGMP-specific 3,5-cyclic phosphodiesterase|cGMP-binding cGMP-specific 3,5-cyclic nucleotide phosphodiesterase|cGMP-specific phosphodiesterase PDE5A2|cGMP-specific phosphodiesterase type 5A|phosphodiesterase 5A, cGMP-specific|phosphodiesterase isozyme 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDE5A, check out the PDE5A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDE5A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PDE5A Antibody Picoband® (A02490-2)
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1 Customer Q&As for Anti-PDE5A Antibody Picoband®
Question
We are currently using anti-PDE5A antibody A02490-2 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
The anti-PDE5A antibody (A02490-2) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-14
