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Product Info Summary
| SKU: | A00802-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA |
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Product info
Product Name
Anti-FGF21 Antibody
SKU/Catalog Number
A00802-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FGF21 Antibody catalog # A00802-1. Tested in ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FGF21 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00802-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human FGF21 recombinant protein (Position: H29-S209).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00802-1 is reactive to FGF21 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
63 kDa
Calculated molecular weight
22300 MW
Background of FGF-21
FGF21 (Fibroblast growth factor 21) is a protein that in humans is encoded by the FGF21 gene, which is also a member of the fibroblast growth factor (FGF) family. The FGF21 gene is mapped on 19q13.33. Using RT-PCR, Fgf21 was expressed in several types of adipose tissue in mice, including subcutaneous and epididymal fat pads and brown adipose tissue. The level of Fgf21 expression in adipose tissue was comparable to that in liver. FGF21 stimulates glucose uptake in adipocytes but not in other cell types. This effect is additive to the activity of insulin. FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, a protein linking FGF receptors to the Ras/MAP kinase pathway. FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents. Changes in Fgf21 expression due to suckling or nutritional manipulations were associated with changes in circulating free fatty acid and ketone body levels. In differentiated mouse brown adipocytes in culture, Fgf21 treatment increased the expression of thermogenic genes, caused higher total and uncoupled respiration, and enhanced glucose oxidation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00802-1 is guaranteed for ELISA Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA Capture (Matched Antibody Pair), 2-8μg/ml
Validation Images & Assay Conditions
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Figure 1. Sandwich ELISA - Recombinant human FGF21 protein standard curve.
Use in combination with reagents from Human FGF21 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0994).
Protein Target Info & Infographic
Gene/Protein Information For FGF21 (Source: Uniprot.org, NCBI)
Gene Name
FGF21
Full Name
Fibroblast growth factor 21
Weight
22300 MW
Superfamily
heparin-binding growth factors family
Alternative Names
Fibroblast growth factor 21;FGF-21;FGF21;UNQ3115/PRO10196; FGF21 fibroblast growth factor 21 fibroblast growth factor 21
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FGF21, check out the FGF21 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FGF21: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FGF21 Antibody (A00802-1)
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-FGF21 Antibody
Question
Is this A00802-1 anti-FGF21 antibody reactive to the isotypes of FGF21?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
The immunogen of A00802-1 anti-FGF21 antibody is E. coli-derived human FGF21 recombinant protein (Position: H29-S209). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-05-04
Question
I was wanting to use your anti-FGF21 antibody for ELISA for human right lobe of liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human right lobe of liver identification?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
As indicated on the product datasheet, A00802-1 anti-FGF21 antibody has been validated for ELISA on human tissues. We have an innovator award program that if you test this antibody and show it works in human right lobe of liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-21
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right lobe of liver using anti-FGF21 antibody A00802-1. Let me know if you need anything else.
D. Carter
Verified customer
Asked: 2020-04-13
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-13
Question
We are currently using anti-FGF21 antibody A00802-1 for human tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
The anti-FGF21 antibody (A00802-1) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-15
Question
I see that the anti-FGF21 antibody A00802-1 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-03-06
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-03-06
Question
Please see the WB image, lot number and protocol we used for right lobe of liver using anti-FGF21 antibody A00802-1. Please let me know if you require anything else.
R. Mitchell
Verified customer
Asked: 2019-01-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-24
Question
We have observed staining in human lung. Any tips? Is anti-FGF21 antibody supposed to stain lung positively?
L. Carter
Verified customer
Asked: 2018-11-13
Answer
From what I have seen in literature lung does express FGF21. From what I have seen in Uniprot.org, FGF21 is expressed in right lobe of liver, lung, among other tissues. Regarding which tissues have FGF21 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-11-13
Question
Do you have a BSA free version of anti-FGF21 antibody A00802-1 available?
B. Anderson
Verified customer
Asked: 2018-09-03
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-FGF21 antibody A00802-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-09-03
Question
Can you help my question with product A00802-1, anti-FGF21 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
D. Jha
Verified customer
Asked: 2018-06-18
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00802-1 anti-FGF21 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-06-18
Question
I am interested in to test anti-FGF21 antibody A00802-1 on human right lobe of liver for research purposes, then I may be interested in using anti-FGF21 antibody A00802-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-04-25
Answer
The products we sell, including anti-FGF21 antibody A00802-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-04-25
Question
Our lab were happy with the WB result of your anti-FGF21 antibody. However we have seen positive staining in lung secreted. using this antibody. Is that expected? Could you tell me where is FGF21 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-01-10
Answer
From what I have seen in literature, lung does express FGF21. Generally FGF21 expresses in secreted. Regarding which tissues have FGF21 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-01-10
Question
Would anti-FGF21 antibody A00802-1 work on monkey ELISA with lung?
M. Parker
Verified customer
Asked: 2017-08-16
Answer
Our lab technicians have not validated anti-FGF21 antibody A00802-1 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-FGF21 antibody A00802-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey lung in ELISA, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-16
Question
Does A00802-1 anti-FGF21 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
T. Wu
Verified customer
Asked: 2016-12-27
Answer
It shows on the product datasheet, A00802-1 anti-FGF21 antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-12-27
Question
Does anti-FGF21 antibody A00802-1 work for ELISA with right lobe of liver?
K. Jones
Verified customer
Asked: 2016-04-11
Answer
According to the expression profile of right lobe of liver, FGF21 is highly expressed in right lobe of liver. So, it is likely that anti-FGF21 antibody A00802-1 will work for ELISA with right lobe of liver.
Boster Scientific Support
Answered: 2016-04-11
Question
Is a blocking peptide available for product anti-FGF21 antibody (A00802-1)?
A. Thomas
Verified customer
Asked: 2013-09-27
Answer
We do provide the blocking peptide for product anti-FGF21 antibody (A00802-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-09-27
