Anti-Hu CD300e Purified Monoclonal Antibody

Product Name

Anti-Hu CD300e Purified Monoclonal Antibody

View all CD300e/LMIR6 Antibodies

SKU/Catalog Number

M11226

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD300e Purified Monoclonal Antibody (Catalog# M11226). Tested in Flow Cytometry, IP, WB application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD300e Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M11226)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

UP-H2

Isotype

Mouse IgG1 kappa

Immunogen

CD300e-HA-transfected cells. The mouse monoclonal antibody UP-H2 recognizes an extracellular epitope on CD300e / IREM-2, a 32 kDa glycoprotein expressed by mature monocytes and peripheral blood myeloid dendritic cells.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

As in case with other anti-CD170 antibodies, this antibody cross-reacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.

Reactive Species

M11226 is reactive to CD300E in Human

Reconstitution

Observed Molecular Weight

42 kDa

Calculated molecular weight

22918 MW

Background of CD300e/LMIR6

CD300e / IREM-2 (immune receptor expressed by myeloid cells 2), also known as CLM2 or LMIR6, is a monomeric transmembrane glycoprotein with a single extracellular immunoglobulin-like domain. Intracellularly it associates with DAP-12, an ITAM-containing adaptor molecule. CD300e is expressed on mature monocytes and peripheral blood myeloid dendritic cells. Its crosslinking leads to release of pro-inflammatory cytokines, and increased expression of activation markers.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M11226 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 1-4 μg/ml.

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

Gene/Protein Information For CD300E (Source: Uniprot.org, NCBI)

Alternative Names

CLM2, CMRF35-A5, LMIR6, IREM-2, PlgR2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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