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Product Info Summary
| SKU: | EK1984 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse CRELD2 ELISA Kit PicoKine®
SKU/Catalog Number
EK1984
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse CRELD2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Creld2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse CRELD2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1984)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: S23-L350
Sensitivity
<50 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1984 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1984 is reactive to Creld2 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1984 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Creld2
Cysteine-rich with EGF-like domain protein 2 is a protein that in humans is encoded by the CRELD2 gene. It is mapped to 15; 15 E3. CRELD2 was identified by searching a sequence database using CRELD1 as the query. Like CRELD1, with which it shares 38% amino acid identity, the deduced 321-amino acid CRELD2 protein contains EGF-like repeats and a highly conserved domain rich in glutamic acid and tryptophan (WE domain). The major distinction between CRELD1 and CRELD2 is that CRELD1 has 2 type III transmembrane domains in its C terminus, while CRELD2 has none. CRELD2 shares 67% and 69% amino acid identity with its Chinese hamster and mouse homologs, respectively.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1984-CAP | Anti-Mouse Creld2 Pre-coated 96-well strip microplate | 1 |
| EK1984-ST | Mouse Creld2 Standard | 2 vials, 10 ng/tube |
| EK1984-DA | Mouse Creld2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.042 | 0.091 | 0.137 | 0.220 | 0.404 | 0.671 | 1.218 | 2.080 |
Data Example Images
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Mouse CRELD2 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Creld2, Dilution ratio of 1:4, concentration in serum and plasma is around 6 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse CRELD2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 214 | 1244 | 5866 | 219 | 1247 | 6512 |
| Standard deviation | 10.06 | 65.93 | 328.50 | 14.67 | 63.60 | 312.58 |
| CV (%) | 4.7% | 5.3% | 5.6% | 6.7% | 5.1% | 4.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Creld2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 214 | 193 | 191 | 207 | 201 | 9.60 | 4.7% |
| Sample 2 | 1244 | 1136 | 1076 | 1247 | 1175 | 72.91 | 6.2% |
| Sample 3 | 5866 | 4866 | 5133 | 5509 | 5343 | 378.38 | 7.0% |
Protein Target Info & Infographic
Gene/Protein Information For Creld2 (Source: Uniprot.Org, NCBI)
Gene Name
Creld2
Full Name
Protein disulfide isomerase Creld2
Weight
38.22kDa
Superfamily
CRELD family
Alternative Names
Cysteine-rich with EGF-like domain protein 2; Creld2 Creld2|5730592L21Rik, C85758|cysteine-rich with EGF-like domains 2|protein disulfide isomerase Creld2|cysteine-rich with EGF-like domain protein 2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Creld2, check out the Creld2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Creld2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse CRELD2 ELISA Kit PicoKine® (EK1984)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Mouse CRELD2 ELISA Kit PicoKine®
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-12-15
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-12-15
Question
Q: if the enzyme conjugated Creld2 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Creld2 antigen?
Verified Customer
Verified customer
Asked: 2020-07-01
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-07-01
Question
Q: is it okay to use citrate plasma as samples in Mouse Creld2 Picokine® ELISA Kit (Catalog # EK1984)?
Verified Customer
Verified customer
Asked: 2020-03-07
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Creld2 causing disruption of its protein structure. Creld2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Creld2 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-03-07
Question
Q: how to analyze ELISA data? I have obtained Creld2 level in plasma.
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-10-08
Question
Q: What is the optimal O.D. value for Creld2 ELISA kit? I performed your Creld2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Creld2 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-01-21
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Creld2 ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of Creld2 that can be declared/interpreted as positive by the above standard is 50pg/ml.
Boster Scientific Support
Answered: 2019-01-21
Question
Q: Can Creld2 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
S. Kaur
Verified customer
Asked: 2017-10-14
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2017-10-14
