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Product Info Summary
| SKU: | A01407 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CPE/Carboxypeptidase E Antibody
View all Carboxypeptidase E/CPE Antibodies
SKU/Catalog Number
A01407
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CPE/Carboxypeptidase E Antibody catalog # A01407. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CPE/Carboxypeptidase E Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01407)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the C-terminal region of human CPE. AA range:427-476
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01407 is reactive to CPE in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
53151 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01407 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
IHC-P, 1:100-300
Validation Images & Assay Conditions
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Western Blot (WB) analysis of Mouse Brain cells using CPE Polyclonal antibody.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Rat Brain, antibody was diluted at 1:100.
Protein Target Info & Infographic
Gene/Protein Information For CPE (Source: Uniprot.org, NCBI)
Gene Name
CPE
Full Name
Carboxypeptidase E
Weight
53151 MW
Superfamily
peptidase M14 family
Alternative Names
Carboxypeptidase E;CPE;3.4.17.10;Carboxypeptidase H;CPH;Enkephalin convertase;Prohormone-processing carboxypeptidase;CPE; CPE CPH carboxypeptidase E carboxypeptidase E|carbocypeptidase E|carboxypeptidase H|cobalt-stimulated chromaffin granule carboxypeptidase|enkephalin convertase|epididymis secretory sperm binding protein|insulin granule-associated carboxypeptidase|prohormone-processing carboxypeptidase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CPE, check out the CPE Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CPE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CPE/Carboxypeptidase E Antibody (A01407)
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No publications found for A01407
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-CPE/Carboxypeptidase E Antibody
Question
Would A01407 anti-CPE/Carboxypeptidase E antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
It shows on the product datasheet, A01407 anti-CPE/Carboxypeptidase E antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-19
Question
Can you help my question with product A01407, anti-CPE/Carboxypeptidase E antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Jackson
Verified customer
Asked: 2019-09-06
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01407 anti-CPE/Carboxypeptidase E antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-06
Question
I see that the anti-CPE/Carboxypeptidase E antibody A01407 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-01-03
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-01-03
Question
I was wanting to use your anti-CPE/Carboxypeptidase E antibody for IHC for human amygdala teratocarcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human amygdala teratocarcinoma identification?
W. Krishna
Verified customer
Asked: 2017-12-21
Answer
It shows on the product datasheet, A01407 anti-CPE/Carboxypeptidase E antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human amygdala teratocarcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-12-21
Question
Will anti-CPE/Carboxypeptidase E antibody A01407 work on pig IHC with brain?
Z. Anderson
Verified customer
Asked: 2015-06-10
Answer
Our lab technicians have not validated anti-CPE/Carboxypeptidase E antibody A01407 on pig. You can run a BLAST between pig and the immunogen sequence of anti-CPE/Carboxypeptidase E antibody A01407 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig brain in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-06-10
Question
Is a blocking peptide available for product anti-CPE/Carboxypeptidase E antibody (A01407)?
T. Evans
Verified customer
Asked: 2013-08-15
Answer
We do provide the blocking peptide for product anti-CPE/Carboxypeptidase E antibody (A01407). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-08-15
