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Product Info Summary
| SKU: | FEK1400 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Mouse Complement Factor H/CFH PicoKine® Quick ELISA Kit
See all Complement Factor H products
SKU/Catalog Number
FEK1400
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Complement Factor H/CFH PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Complement Factor H/CFH in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Complement Factor H/CFH PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1400)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: S875-V1252
Sensitivity
<20 pg/ml
Assay Range
0.78 ng/ml - 50 ng/ml
Standard Dilution Instructions
See datasheet of FEK1400 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK1400 is reactive to CFH in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Application Guarantee
FEK1400 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Complement Factor H
Complement factor H (CFH), originally known as beta-1H globulin, is a serum glycoprotein that regulates the function of the alternativecomplement pathway in fluid phase and on cellular surfaces. It binds to C3b, accelerates the decay of the alternative pathway convertase C3bBb, and also acts as a cofactor for complement factor I, another C3b inhibitor. The CFH gene is located on chromosome 1q32-q32.1 within a cluster of genes encoding the regulatory complement components of the activation of C3 (RCA for 'regulators of complement activation'). This gene cluster includes decay-accelerating factor (DAF), C4-binding protein (C4BPA and C4BPB), and the factor H-related genes CFHR1, CFHR2, CFHR3, CFHR4, and CFHR5, among others. The gene family has arisen by multiple duplication events.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK1400-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK1400-ST | Mouse Complement Factor H Standard | 2 | 100 ng/tube |
| FEK1400-A | Mouse CFH Antibody A | 1 | 3ml |
| FEK1400-B | Mouse CFH Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
| O.D. | 0.002 | 0.313 | 0.609 | 1.052 | 1.626 | 2.217 | 2.290 | 2.478 |
Data Example Images
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Mouse CFH PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 1344 | 6741 | 29641 | 1352 | 6941 | 26413 |
| Standard deviation | 72.58 | 242.68 | 1304.2 | 51.38 | 423.4 | 1558.37 |
| CV(%) | 5.4% | 3.6% | 4.4% | 3.8% | 6.1% | 5.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Complement Factor H in ELISA kits from four different production batches/lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1344 | 1258 | 1323 | 1429 | 1339 | 84.33 | 6.3% |
| Sample 2 | 6741 | 6541 | 5974 | 6641 | 6474 | 407.88 | 6.3% |
| Sample 3 | 29641 | 26474 | 24165 | 26852 | 26783 | 1553.41 | 5.8% |
Protein Target Info & Infographic
Gene/Protein Information For CFH (Source: Uniprot.Org, NCBI)
Gene Name
CFH
Full Name
Complement factor H
Weight
Alternative Names
adrenomedullin binding protein; age-related maculopathy susceptibility 1; AHUS1; AMBP1; ARMD4; ARMS1; beta-1H; beta-1-H-globulin ; beta-1-H-globulin; CFH; CFHL3; Complement Factor H; factor H; factor H-like 1; FH; FHL1; H factor 1 (complement); H factor 1; H factor 2 (complement); HF; HF1; HF1ARMS1; HF2; HUS; HUSMGC88246 CFH AHUS1, AMBP1, ARMD4, ARMS1L3, FH, FHL1, HF, HF1, HF2, HUS, CFH complement factor H complement factor H|H factor 1 (complement)|H factor 2 (complement)|adrenomedullin binding protein|age-related maculopathy susceptibility 1|beta-1-H-globulin|beta-1H|factor H|factor H-like 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CFH, check out the CFH Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CFH: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Complement Factor H/CFH PicoKine® Quick ELISA Kit (FEK1400)
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